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Birth of MHC-defined rhesus monkeys produced by assisted reproductive technology

机译：通过辅助生殖技术生产的MHC恒河猴的诞生

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One of the best animal approaches for testing HIV vaccines is the challenge of vaccinated rhesus macaques with SHIV or SIV. Production of rhesus macaques in which all of the MHC class I and II alleles are known represents an opportunity to characterize the entire immune response to SIV and should be an invaluable resource for understanding pathogenesis and vaccine-induced immune responses. Unfortunately, there are few MHC-defined rhesus macaques available for vaccine research. Selective breeding supports the production of limited numbers of macaques that express particular MHC class I alleles. If both parents express the allele of interest, only three quarters of the offspring will express the same allele, However, assisted reproductive technologies, such as in vitro fertilization (IVF) and embryo transfer, can be used for production of MHC-defined macaques, expressing multiple MHC class I and II molecules for which SIV peptides. tetramers and ELISPOT assays exist. Here, we report the birth of MHC-defined rhesus monkeys produced by assisted reproductive technology. Continued improvements in assisted reproductive technologies in rhesus monkeys will enable us to develop a unique prototypic animal production program for the creation of MHC-defined and genetically-identical monkeys for vaccine research.
机译：测试HIV疫苗的最佳动物方法之一是接种SHIV或SIV的恒河猴的挑战。已知其中所有MHC I类和II类MHC等位基因的恒河猴的生产代表了表征针对SIV的整个免疫反应的机会，并且应该是了解发病机理和疫苗诱导的免疫反应的宝贵资源。不幸的是，很少有MHC定义的恒河猕猴可用于疫苗研究。选择性育种支持生产有限数量的猕猴，它们表达特定的MHC I类等位基因。如果父母双方都表达了感兴趣的等位基因，那么只有四分之三的后代会表达相同的等位基因。但是，辅助生殖技术（例如体外受精（IVF）和胚胎移植）可以用于生产MHC限定的猕猴，表达多个具有SIV肽的MHC I和II类分子。存在四聚体和ELISPOT分析。在这里，我们报告了由辅助生殖技术生产的MHC定义的恒河猴的出生。恒河猴辅助生殖技术的不断改进将使我们能够开发独特的原型动物生产计划，以创建MHC定义的和遗传相同的猴子进行疫苗研究。

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