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首页> 外文期刊>Vaccine >Human airway epithelial cells present antigen to influenza virus-specific CD8+ CTL inefficiently after incubation with viral protein together with ISCOMATRIX
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Human airway epithelial cells present antigen to influenza virus-specific CD8+ CTL inefficiently after incubation with viral protein together with ISCOMATRIX

机译：与病毒蛋白和ISCOMATRIX一起孵育后，人气道上皮细胞无法有效地将抗原呈递给流感病毒特异性CD8 + CTL

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In the present paper, an in vitro model was established in which the interaction between influenza virus-specific CD8+ T cells and human airway epithelial cells can be studied. To this end, the human lung epithelial cell line A549 was transduced with the HLA-A*0201 gene. This MHC class I allele is involved in the presentation of the immunodominant M158-66 cytotoxic T lymphocyte (CTL) epitope of the influenza A virus matrix protein. The A549-HLA-A2 cells and a CD8+ T cell clone specific for the M158-66 epitope were used to evaluate ISCOMATRIX (IMX), which is considered a potential mucosal adjuvant for influenza vaccines, for its capacity to activate virus-specific CTL after incubation with epithelial cells. It was found that virus infected epithelial cells activated virus-specific CTL efficiently. However, incubation of epithelial cells with ISCOMATRIX and recombinant M1 protein activated CD8+ T cells inefficiently, unlike the incubation of C1R cells expressing a HLA-A2 trans gene or HLA-A2+ B-lymphoblastoid cells with these reagents. It was concluded that this lack of antigen presentation by epithelial cells indicate that these cells are not subject to killing by virus-specific CTL upon instillation with ISCOMATRIX-based vaccines, which may be a favorable property of mucosal vaccines.
机译：在本文中，建立了一个体外模型，在其中可以研究流感病毒特异性CD8 + T细胞与人气道上皮细胞之间的相互作用。为此，用HLA-A * 0201基因转导了人肺上皮细胞系A549。该MHC I类等位基因参与了甲型流感病毒基质蛋白的免疫优势M158-66细胞毒性T淋巴细胞（CTL）表位的呈递。针对M158-66表位特异的A549-HLA-A2细胞和CD8 + T细胞克隆用于评估ISCOMATRIX（IMX），它被认为是流感疫苗的潜在粘膜佐剂，具有在感染后激活病毒特异性CTL的能力。与上皮细胞一起孵育。发现病毒感染的上皮细胞有效地激活了病毒特异性CTL。但是，与ISCOMATRIX和重组M1蛋白一起孵育上皮细胞不能有效激活CD8 + T细胞，这与用这些试剂孵育表达HLA-A2反基因或HLA-A2 + B淋巴母细胞的C1R细胞不同。结论是上皮细胞缺乏抗原呈递，表明这些细胞在灌输基于ISCOMATRIX的疫苗后不会受到病毒特异性CTL的杀死，这可能是粘膜疫苗的有利特性。

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