A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Lomada Dakshayani; Gambhira Ratish; Nehete Pramod N; Guhad Faisal A; Chopra Ashok K; Peterson Johnny W; Sastry K Jagannadha

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A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

机译：缺乏ADP-核糖基化活性的霍乱毒素的两个密码子突变体作为引发佐剂和全身细胞对肽抗原的免疫应答的有效佐剂

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Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses. Copyright 2004 Published by Elsevier Ltd.

机译：肽抗原疫苗接种是抵抗粘膜病毒感染的有效策略。我们测试了霍乱毒素（CT-2 *）的两密码子突变体，该突变体缺乏ADP-核糖基化活性和毒性，作为小鼠鼻内免疫T细胞表位肽的粘膜佐剂。在包括鼻，肠和阴道相关淋巴组织在内的全身和粘膜区室中观察到与TH1细胞因子产生相关的辅助诱导和细胞毒性T淋巴细胞反应的有效诱导。肽抗原和CT-2 *的单次或多次给药诱导了强大的记忆免疫力，而没有耐受性。这些结果证明CT-2 *是用于引发抗原特异性细胞免疫应答的合适粘膜佐剂。版权所有2004，由Elsevier Ltd.发布。

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《Vaccine》 |2004年第4期|共11页
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Lomada Dakshayani; Gambhira Ratish; Nehete Pramod N; Guhad Faisal A; Chopra Ashok K; Peterson Johnny W; Sastry K Jagannadha;
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中图分类卫生标准、卫生检查、医药管理;
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1. A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens [J] . Lomada Dakshayani, Gambhira Ratish, Nehete Pramod N, Vaccine . 2004,第4期

机译：缺乏ADP-核糖基化活性的霍乱毒素的两个密码子突变体作为引发佐剂和全身细胞对肽抗原的免疫应答的有效佐剂
2. Mucosal and systemic adjuvant effects of cholera toxin and Cry1Ac protoxin on the specific antibody response to HIV-1 C4/V3 peptides are different and depend on the antigen co-administered. [J] . Esquivel Perez R, Moreno Fierros L Viral immunology . 2005,第4期

机译：霍乱毒素和Cry1Ac毒素对HIV-1 C4 / V3肽的特异性抗体应答的黏膜和全身佐剂作用是不同的，取决于共同施用的抗原。
3. Heteropentameric Cholera Toxin B Subunit Chimeric Molecules Genetically Fused to a Vaccine Antigen Induce Systemic and Mucosal Immune Responses: a Potential New Strategy To Target Recombinant Vaccine Antigens to Mucosal Immune Systems [J] . Tetsuya Harakuni, Hideki Sugawa, Ai Komesu, Infection and immunity . 2005,第9期

机译：遗传融合到疫苗抗原的异源性霍乱毒素B亚基嵌合分子诱导全身和粘膜免疫反应：靶向重组疫苗抗原对粘膜免疫系统的潜在新策略。
4. Intranasal vaccination with a lipopeptide containing a minimal, conserved M-protein derived peptide and a self-adjuvanting lipid induces both systemic and mucosal immune responses in mice [C] . Michael R. Batzloff, Jon Hartas, Weiguang Zeng, Lancefield International Symposium on Streptococci and Streptococcal Diseases . 2006

机译：用含有最小，保守的M蛋白衍生的肽和自佐剂脂质的脂肽接种疫苗接种诱导小鼠的全身和粘膜免疫应答
5. Modulation of the immune response at systemic and mucosal sites by DNA vaccine adjuvants in HIV and influenza models. [D] . Kraynyak, Kimberly Ann. 2009

机译：DNA疫苗佐剂在HIV和流感模型中对全身和粘膜部位免疫应答的调节。
6. Note: Adjuvant Activity of a Nontoxic Mutant of Escherichia coli Heat-Labile Enterotoxin on Systemic and Mucosal Immune Responses Elicited against a Heterologous Antigen Carried by a Live Salmonella enterica Serovar Typhimurium Vaccine Strain [O] . Heloisa C. R. Guillobel, Joana I. Carinhanha, Lucia Cárdenas, 2000

机译：注意：大肠杆菌热不稳定肠毒素的无毒突变体对活肠沙门氏菌血清型鼠伤寒疫苗株携带的异源抗原引起的全身和粘膜免疫反应的佐剂活性。
7. Adjuvant Activity of a Nontoxic Mutant of Escherichia coli Heat-Labile Enterotoxin on Systemic and Mucosal Immune Responses Elicited against a Heterologous Antigen Carried by a Live Salmonella enterica Serovar Typhimurium Vaccine Strain [O] . Guillobel, Heloisa C. R., Carinhanha, Joana I., Cárdenas, Lucia, 2000

机译：大肠杆菌热不稳定肠毒素的无毒突变体对活肠沙门氏菌血清型鼠伤寒疫苗株携带的异源抗原引起的全身和粘膜免疫应答的佐剂活性。
8. Development of Carriers and Adjuvants for Use with Peptides to Induce Mucosal and Systemic Immunity against Biologic Toxins. [R] . Arnon, R. 1993

机译：开发用于肽的载体和佐剂，以诱导针对生物毒素的粘膜和系统免疫。

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

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