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The efficacy of electroporated plasmid vaccines correlates with long-term antigen production in vivo

机译：电穿孔质粒疫苗的功效与体内长期抗原产生相关

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We sought to determine whether in vivo electroporation might overcome the requirement for strong transcriptional promoters in plasmid vaccines. We tested plasmid vaccines containing viral promoters that exhibited a broad range of transcriptional activities by in vitro assay. In contrast to the in vitro observations, no difference in gene expression was measured in vivo 24 h following injection when the plasmids were introduced via electroporation. Despite the similarities in gene expression in vivo, the cellular and Immoral responses elicited by these plasmid vaccines were dramatically different and correlated more closely with the promoter activity measured in vitro. When antigen production in vivo was measured over a longer period following injection, significant differences in gene expression became apparent after 2-3 days and the differences in gene expression at day 7 correlated well with plasmid immunogenicity. These studies reconfirm the importance of antigen productiori for effective plasmid vaccination and demonstrate that the duration of gene expression should also be considered when designing plasmid vaccines.
机译：我们试图确定体内电穿孔是否可以克服质粒疫苗中强转录启动子的要求。我们通过体外试验测试了包含病毒启动子的质粒疫苗，该病毒启动子表现出广泛的转录活性。与体外观察相反，当通过电穿孔引入质粒时，在注射后24小时体内没有测量到基因表达的差异。尽管体内基因表达相似，但是这些质粒疫苗引发的细胞和不育反应却截然不同，并且与体外测量的启动子活性更紧密相关。当在注射后较长时间测量体内抗原产生时，在2-3天后基因表达的显着差异变得明显，并且在第7天基因表达的差异与质粒免疫原性良好相关。这些研究证实了抗原产生对于有效的质粒疫苗接种的重要性，并证明在设计质粒疫苗时也应考虑基因表达的持续时间。

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