...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus
【24h】

Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus

机译：猪分枝杆菌卡介苗表达猪繁殖与呼吸综合征病毒GP5和M蛋白的截短形式的免疫反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pigs were immunised with recombinant BCG (rBCG) expressing a truncated form of GP5 (lacking the first 30 NH2-terminal residues) (rBCGGP5) and M protein (rBCGM) of porcine reproductive and respiratory syndrome virus (PRRSV). At 30 days post-inoculation (dpi), pigs inoculated with rBCGGP5 and rBCGM developed a specific humoral immune response against the viral proteins, as detected by commercial ELISA and Western blot tests, and at 60 dpi, three out of five animals developed neutralizing antibodies with titers ranging from 1:4 to 1:8. At 67 dpi, an IFN-gamma response against BCG antigens, but not against the viral proteins, was detected by ELISPOT in inoculated pigs. Following challenge with a pathogenic strain of PRRSV, pigs inoculated with rBCG showed lower (P < 0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection
机译：用重组BCG（rBCG）免疫猪，该蛋白表达截短形式的GP5（缺少前30个NH2末端残基）（rBCGGP5）和猪繁殖与呼吸综合征病毒（PRRSV）的M蛋白（rBCGM）。接种后30天（dpi），用商业ELISA和Western印迹试验检测到，接种rBCGGP5和rBCGM的猪对病毒蛋白产生了特异性的体液免疫反应，在60 dpi时，五分之三的动物产生了中和抗体滴度范围为1：4至1：8。在67 dpi时，通过ELISPOT在接种的猪中检测到了针对BCG抗原的IFN-γ反应，但未针对病毒蛋白。用致病性PRRSV株攻击后，接种rBCG的猪在支气管淋巴结中的温度，病毒血症和病毒载量比对照动物低（P <0.05），这表明已建立了针对PRRSV感染的部分保护

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号