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Immunogenicity of the Brucella melitensis recombinant ribosome recycling factor-homologous protein and its cDNA

机译：布鲁氏菌重组核糖体回收因子同源蛋白及其cDNA的免疫原性

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摘要

A study was conducted to evaluate the immunogenicity of the Brucella melitensis ribosome recycling factor (RRF)-homologous protein (CP24). The CP24 gene was cloned, expressed in Escherichia coli and purified. The resulting purified recombinant protein (rCP24) produced delayed-type hypersensitivity (DTH) reactions in B. melitensis-infected mice but not in naive controls. Thus, we decided to characterise the immune responses generated with DNA vaccination (pcDNACP24) or immunisation with the rCP24 in adjuvant. Animals injected with pcDNACP24 exhibited a dominance of IgG2a to IgG1 while mice injected with rCP24 developed a higher response of IgG1 than IgG2a. Both immunisation protocols were capable of eliciting CP24-specific gamma interferon (IFN-gamma) producing cells. Spleen cells from peDNACP24-immunised mice did not produce interleukin (IL)-4, IL-10 or up-regulation of IL-2 mRNA. Cells from rCP24-immunised mice produced IL-10, up-regulated IL-2 mRNA but did not produce IL-4. Neither immunisation with purified CP24 nor injection of pcDNACP24 protected mice against challenge with live smooth B. melitensis. However, the potential of CP24 for a Brucella diagnostic test based on an in vitro antigen (Ag)-specific IFN-gamma production or DTH test would be worth testing.
机译：进行了一项研究，以评估布鲁氏菌核糖体回收因子（RRF）同源蛋白（CP24）的免疫原性。克隆CP24基因，在大肠杆菌中表达并纯化。所得的纯化重组蛋白（rCP24）在感染B. melitensis的小鼠中产生了迟发型超敏反应（DTH），但未在幼稚对照中产生。因此，我们决定表征佐剂中DNA疫苗接种（pcDNACP24）或rCP24免疫产生的免疫应答。注射pcDNACP24的动物表现出IgG2a对IgG1的优势，而注射rCP24的小鼠比IgG2a产生更高的IgG1反应。两种免疫方案均能够诱导产生CP24特异性γ干扰素（IFN-γ）的细胞。 peDNACP24免疫小鼠的脾细胞不产生白介素（IL）-4，IL-10或IL-2 mRNA的上调。来自经rCP24免疫的小鼠的细胞产生IL-10，上调的IL-2 mRNA，但不产生IL-4。用纯化的CP24免疫和注射pcDNACP24都不能保护小鼠免受活的光滑B. melitensis的攻击。但是，CP24用于基于体外抗原（Ag）特异性IFN-γ产生或DTH测试的布鲁氏菌诊断测试的潜力值得测试。

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