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Genetic and phenotypic analysis of reassortants of high growth and low growth strains of influenza B virus

机译：乙型流感病毒高生长株和低生长株重组子的遗传和表型分析

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摘要

The yield of influenza virus in eggs is critical to influenza vaccine production and availability, but the contribution of specific genes to the growth properties of influenza B viruses is not well understood. Influenza B/Beijing/184/93 and B/Shangdong/7/97 were chosen for study because B/Shangdong/7/97 replicated to several fold higher titers in eggs than B/Beijing/184/93 as demonstrated by hemagglutination titers and EID50. A reassortant with the HA, NP and PB2 genes from B/Beijing/184/93 and all other genes from B/Shangdong/7/97 had the high growth phenotype of B/Shangdong/7/97 in eggs, which suggests that NS, M, NA, PBIor PA, or a combination of these genes derived from B/Shangdong/7/97 were needed for the high growth phenotype of the reassortants. A high degree of homology was found among the genetic sequences of B/Beijing/184/93, B/Shangdong/7/97, and other influenza B viruses. However, differences potentially related to growth characteristics were suggested by analysis of the deduced amino acid (AA) sequences of four genes: NS (NS1, NS2), M (BM2). NA (NA, NB) and PB1. The studies identify multiple genes that may affect growth of influenza B viruses in eggs
机译：鸡蛋中流感病毒的产量对于流感疫苗的生产和可获得性至关重要，但是尚不清楚特定基因对乙型流感病毒生长特性的贡献。选择B / Beijing / 184/93和B / Shangdong / 7/97流感进行研究是因为B / Shangdong / 7/97在鸡蛋中的滴度比B / Beijing / 184/93高出几倍，如血凝滴度和EID50。与来自B / Beijing / 184/93的HA，NP和PB2基因以及来自B / Shangdong / 7/97的所有其他基因的重配体在鸡蛋中具有B / Shangdong / 7/97的高生长表型，这表明NS重配体的高生长表型需要M，NA，PBI或PA或M，NA，PBI或PA / S / dong / 7/97衍生的这些基因的组合。在B / Beijing / 184/93，B / Shangdong / 7/97和其他B型流感病毒的基因序列之间发现了高度同源性。但是，通过分析四个基因的推导氨基酸（AA）序列，可以暗示与生长特性相关的差异：NS（NS1，NS2），M（BM2）。 NA（NA，NB）和PB1。这项研究确定了可能影响鸡蛋中乙型流感病毒生长的多个基因

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