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Cytokine production patterns and antibody response to measles vaccine

机译：细胞因子的生产方式和对麻疹疫苗的抗体反应

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Cytokines play an important role in the immune response to live measles virus immunization. To gain further insight into the cytokine production profile in response to measles vaccination, we studied interferon-gamma (IFN-gamma), tumor necrosis factor (TNF-alpha), soluble IL-2 receptor (sIL-2R), interleukin-2 (IL-2), interleukin-4 (IL-4), and interleukin-6 (IL-6) in both supernatants from peripheral blood mononuclear cells (PBMC) stimulated with phytohaemagglutinin (PHA), and plasma. We enrolled 57 healthy infants and children residing in an area where no measles virus circulated in their lifetimes. Overall analysis of cytokines in supernatants from PBMC showed that a predominant Th1 cytokine pattern occurs after the second dose of measles immunization. However, plasma levels of Th1 cytokines (IFN-gamma, sIL-2R and TNF-alpha) were preferentially activated by measles virus after the first dose of measles vaccination. Median IFN-gamma plasma levels were 1.73 pg/ml for infants compared to 0.63 pg/ml for older children (P = 0.003). These data suggest that after the first and the second dose of measles virus immunization, there is a predominant Th1-type directed immune response, but the Th1 cytokine pattern seems to be stronger in previously unvaccinated children. There was no correlation between cytokine production by PBMC supernatants after PHA stimulation and circulating levels of plasma cytokines. No relationship was found between any specific cytokine level and measles antibody level.
机译：细胞因子在对活麻疹病毒免疫的免疫反应中起重要作用。为了进一步了解麻疹疫苗接种后细胞因子的产生情况，我们研究了干扰素-γ（IFN-γ），肿瘤坏死因子（TNF-α），可溶性IL-2受体（sIL-2R），白介素-2（ IL-2），白介素4（IL-4）和白介素6（IL-6）都来自用植物血凝素（PHA）刺激的外周血单个核细胞（PBMC）和血浆中的两种上清液。我们招募了57名健康的婴幼儿，他们生活在一生中都没有传播麻疹病毒的地区。 PBMC上清液中细胞因子的总体分析表明，在第二次麻疹免疫后出现了主要的Th1细胞因子模式。但是，首次接种麻疹疫苗后，麻疹病毒优先激活了Th1细胞因子（IFN-γ，sIL-2R和TNF-α）的血浆水平。婴儿的中位IFN-γ血浆水平为1.73 pg / ml，而大龄儿童为0.63 pg / ml（P = 0.003）。这些数据表明，在第一次和第二次麻疹病毒免疫后，主要存在Th1型定向免疫反应，但在以前未接种疫苗的儿童中，Th1细胞因子模式似乎更强。在PHA刺激后，PBMC上清液产生的细胞因子与血浆细胞因子的循环水平之间没有相关性。在任何特定的细胞因子水平和麻疹抗体水平之间均未发现相关性。

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