Determination of the coding and non-coding nucleotide sequences of genuine Edmonston-Zagreb master seed and current working seed lot

Baricevic M; Forcic D; Gulija TK; Jug R; Mazuran R

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Determination of the coding and non-coding nucleotide sequences of genuine Edmonston-Zagreb master seed and current working seed lot

机译：真正的埃德蒙斯顿-萨格勒布主种子和当前工作种子批次的编码和非编码核苷酸序列的确定

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To confirm the genetic stability of the Edmonston-Zagreb vaccine strain, we determined and compared the nucleotide sequences of genuine Edmonston-Zagreb master seed (EZ D22) and current working seed lot (EZ D24 2/99). Sequence analysis and comparison of the two sequences confirmed that these two sequences are the same at the molecular level. The obtained sequences were also compared to reference strains, i.e. Edmonston wild-type (Edmonston Wt) AF266288 and Edmonston-Zagreb (EZ) AF266290 vaccine strain. The sequence of EZ D22 differed from the Edmonston Wt in 32 nucleotides. EZ D22 differed from EZ AF266290 in six nucleotides. Coding substitution at position 441 and two silent substitutions at positions 11999 and 14612 in the L gene are unique to EZ D22. The differences found between EZ from different sources can be a good reason for periodical sequence analysis of the same strain in the hands of different manufactures.

机译：为了确认Edmonston-Zagreb疫苗株的遗传稳定性，我们确定并比较了真正的Edmonston-Zagreb主种子（EZ D22）和当前工作种子批次（EZ D24 2/99）的核苷酸序列。序列分析和两个序列的比较证实，这两个序列在分子水平上是相同的。还将获得的序列与参考菌株，即埃德蒙斯顿野生型（Edmonston Wt）AF266288和埃德蒙斯顿-萨格勒布（EZ）AF266290疫苗株进行比较。 EZ D22的序列与Edmonston Wt有32个核苷酸的差异。 EZ D22在六个核苷酸上与EZ AF266290不同。 EZ D22在L基因中的441位编码替换和11999和14612位的两个沉默替换是唯一的。来自不同来源的EZ之间发现的差异可能是对不同制造商手中的同一菌株进行定期序列分析的很好原因。

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《Vaccine》 |2005年第8期|共7页
作者
Baricevic M; Forcic D; Gulija TK; Jug R; Mazuran R;
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中图分类卫生标准、卫生检查、医药管理;
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1. Determination of the coding and non-coding nucleotide sequences of genuine Edmonston-Zagreb master seed and current working seed lot [J] . Baricevic M, Forcic D, Gulija TK, Vaccine . 2005,第8期

机译：真正的埃德蒙斯顿-萨格勒布主种子和当前工作种子批次的编码和非编码核苷酸序列的确定
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Determination of the coding and non-coding nucleotide sequences of genuine Edmonston-Zagreb master seed and current working seed lot

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