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首页> 外文期刊>Vaccine >A serine protease inhibitor (serpin) from Haemaphysalis longicornis as an anti-tick vaccine
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A serine protease inhibitor (serpin) from Haemaphysalis longicornis as an anti-tick vaccine

机译：一种来自长血Ha的丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂）作为抗vaccine虫疫苗

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The application of anti-tick vaccine has been shown to be the most promising alternative tick control strategy compared to the current use of acaricides that suffer from a number of limitations. The success of this strategy is dependent on the cloning, and characterization of tick molecules involved in the mediation of tick central physiological roles. Rapid amplification of the cDNA ends (RACE) and primers designed based on a conserved serpin amino acid motif (NAVYFKG) were used to clone a cDNA with high similarity in the reactive center loop (RCL) to representative serpin, heparin cofactor II. We have named this novel gene as Haemaphysalis longicornis serpin-2 (HLS2). RT-PCR analysis showed that HLS2 mRNA transcripts are not expressed in salivary glands but in hemolymph by feeding ticks. HLS2 was not introduced into the bite site as measured by Western blot analysis. The activated partial thromboplastin time (APTT) and the thrombin inhibitory assay using recombinant HLS2 (rHLS2) demonstrated prolonged coagulation time and inhibition of thrombin activity. These results suggested that HLS2 is present only in hemolymph of the feeding ticks and the function of HLS2 is homeostasis in tick physiological compartment. Vaccination of rabbits with rHLS2 conferred protective immunity against ticks, resulting in 44.6 and 43.0% mortality in nymphal and adult ticks, respectively. These results show that rHLS2 could be an important candidate as a component of a cocktail anti-tick vaccine.
机译：与目前使用的受许多限制的杀螨剂相比，抗ick虫疫苗的应用已被证明是最有希望的promising虫控制策略。该策略的成功取决于克隆和参与tick中枢生理作用介导的tick分子的表征。 cDNA末端的快速扩增（RACE）和基于保守的丝氨酸蛋白酶抑制剂氨基酸基序（NAVYFKG）设计的引物被用于克隆一个与代表的丝氨酸蛋白酶抑制剂肝素辅因子II具有高度相似性的cDNA。我们已将此新基因命名为Haemaphysalis longicornis serpin-2（HLS2）。 RT-PCR分析表明，HLS2 mRNA的转录本在唾液腺中不表达，而在血淋巴中以feeding为食。如通过蛋白质印迹分析所测量的，HLS2未引入咬合位点。激活的部分凝血活酶时间（APTT）和使用重组HLS2（rHLS2）进行的凝血酶抑制试验证明了凝血时间的延长和凝血酶活性的抑制。这些结果表明HLS2仅存在于饲养tick的血淋巴中，并且HLS2的功能是tick生理区室中的稳态。用rHLS2进行的兔子疫苗接种可提供抗tick虫的保护性免疫，若虫和成年s虫的死亡率分别为44.6％和43.0％。这些结果表明，rHLS2可能是鸡尾酒抗t虫疫苗的重要组成部分。

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