...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV
【24h】

Single epitope mucosal vaccine delivered via immuno-stimulating complexes induces low level of immunity against simian-HIV

机译：通过免疫刺激复合物递送的单个表位粘膜疫苗诱导了针对猿猴-HIV的低水平免疫

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The difficulty in developing an effective vaccine to contain the HIV/AIDS epidemic coupled with the fact that primary HIV-1 infection typically occurs via mucosal sites has increased emphasis on vaccine approaches that protect at mucosal surfaces. In this study we employed HIV and simian-HIV (SHIV)-derived T helper (Th) and cytotoxic T lymphocyte (CTL) single epitopes incorporated into immuno-stimulating complexes (ISCOM) as a candidate immunogens. Immunized rhesus macaques (Macaca mulatta) were challenged with CCR5-tropic SHIV(SF162p4). On the day of challenge, low levels of virus-neutralizing antibodies (Ab) and CTLs were detected in ISCOM-immunized macaques. Greater than 10(5) viral RNA copies per ml of plasma in 2/5 immunized and 3/4 control macaques were detected within 3 weeks post-challenge. Depletion of CD4+ T cells from gut-associated lymphoid tissues (GALT) was observed by post-challenge day (PCD) 14 in all macaques regardless immunization. Nonetheless, lower viral loads and relatively better preservation of peripheral CD4+ T cells following the SHIV infection was observed in ISCOM-immunized macaques. We predict that if coadministered with additional epitopes and/or more efficacious mucosal delivery system or route, HIV/SIV-derived peptide vaccines may have potential to elicit heterologous protection.
机译：难以开发出有效的疫苗来遏制HIV / AIDS的流行，再加上通常通过粘膜部位发生原发性HIV-1感染这一事实，使人们更加重视在粘膜表面进行保护的疫苗方法。在这项研究中，我们采用了HIV和猿猴HIV（SHIV）衍生的T辅助物（Th）和细胞毒性T淋巴细胞（CTL）单一表位作为免疫原性候选物掺入免疫刺激复合物（ISCOM）中。免疫恒河猴（猕猴）用CCR5嗜性SHIV（SF162p4）攻击。攻击当天，在ISCOM免疫的猕猴中检测到低水平的病毒中和抗体（Ab）和CTL。在攻击后3周内，在2/5免疫的猕猴和3/4对照猕猴中，每毫升血浆中的RNA拷贝数超过10（5）。在攻击后第14天（PCD）观察到，所有猕猴的肠道相关淋巴样组织（GALT）的CD4 + T细胞均已耗尽，无论是否免疫。但是，在ISCOM免疫的猕猴中，观察到SHIV感染后病毒载量较低，并且外周CD4 + T细胞的保存相对较好。我们预测，如果与其他表位和/或更有效的粘膜递送系统或途径共同给药，HIV / SIV衍生的肽疫苗可能具有引起异源保护的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号