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首页> 外文期刊>Vaccine >Protective immune responses induced by different recombinant vaccine regimes to Rift Valley fever
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Protective immune responses induced by different recombinant vaccine regimes to Rift Valley fever

机译：不同重组疫苗方案诱导的裂谷热保护性免疫反应

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摘要

The glycoprotein (GP) and nucleocapsid (NC) genes of Rift Valley fever virus (RVFV) were expressed in different expression systems and were evaluated for their ability to protect mice from virulent challenge using a prime-boost regime. Mice vaccinated with a lumpy skin disease virus-vectored recombinant vaccine (rLSDV-RVFV) expressing the two RVFV glycoproteins (G1 and G2) developed neutralising antibodies and were fully protected when challenged, as were those vaccinated with a crude extract of truncated G2 glycoprotein (tG2). By contrast mice vaccinated with a DNA vaccine expressing G1 and G2 did not sero-convert with only 20% of them surviving challenge. Mice vaccinated with the DNA vaccine and boosted with rLSDV-RVFV also failed to sero-convert but 40% survived challenge. Surprisingly, although none of the mice immunised with the purified NC protein sero-converted, 60% of them survived virulent challenge. The rLSDV-RVFV construct was then further evaluated in sheep for its dual protective abilities against RVFV and sheeppox virus (SPV). Vaccinated sheep sero-converted for both viruses and were protected against RVFV challenge, however, neither the immunised or negative control animals showed any significant reactions to the virulent SPV challenge.
机译：裂谷热病毒（RVFV）的糖蛋白（GP）和核衣壳（NC）基因在不同的表达系统中表达，并通过初免-加强方案评估了它们保护小鼠免受强毒攻击的能力。用表达两种RVFV糖蛋白（G1和G2）的块状皮肤病病毒载体重组疫苗（rLSDV-RVFV）接种的小鼠发展出中和抗体，并在受到攻击时受到完全保护，接种了截短的G2糖蛋白粗提物的小鼠（ tG2）。相比之下，接种了表达G1和G2的DNA疫苗的小鼠没有发生血清转化，只有20％的小鼠存活了攻击。用DNA疫苗接种疫苗并用rLSDV-RVFV加强免疫的小鼠也无法进行血清转化，但40％的小鼠在攻击后仍然存活。出人意料的是，尽管没有小鼠用血清转化的纯化的NC蛋白进行免疫，但是其中有60％的小鼠存活了有力的攻击。然后在绵羊中进一步评估rLSDV-RVFV构建体对RVFV和羊痘病毒（SPV）的双重保护能力。疫苗接种的绵羊对两种病毒都进行了血清转化，并且可以抵抗RVFV攻击，但是，免疫或阴性对照动物均未显示出对有毒SPV攻击的任何显着反应。

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