...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Interfering vaccine (defective interfering influenza A virus) protects ferrets from influenza, and allows them to develop solid immunity to reinfection
【24h】

Interfering vaccine (defective interfering influenza A virus) protects ferrets from influenza, and allows them to develop solid immunity to reinfection

机译：干扰疫苗（有缺陷的干扰性甲型流感病毒）可以保护雪貂免受流感的侵袭，并使它们对再感染具有牢固的免疫力

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Defective interfering (DI) virus RNAs result from major deletions in full-length viral RNAs that occur spontaneously during de novo RNA synthesis. These RNAs are packaged into virions that are by definition non-infectious, and are delivered to cells normally targeted by the virion. DI RNAs can only replicate with the aid of a coinfecting infectious helper virus, but the small size of DI RNA allows more copies of it to be made than of its full-length counterpart, so the cell produces defective virions in place of infectious progeny. In line with this scenario, the expected lethal disease in an influenza A virus-mouse model is made subclinical by administration of DI virus, but animals develop solid immunity to the infecting virus. Hence DI virus has been called an 'interfering vaccine'. Because interfering vaccine acts intracellularly and at a molecular level, it should be effective against all influenza A viruses regardless of subtype. Here we have used the ferret, widely acknowledged as the best model for human influenza. We show that an interfering vaccine with defective RNAs from an H3N8 virus almost completely abolished clinical disease caused by A/Sydney/5/97 (H3N2), with abrogation of fever and significant reductions in clinical signs of illness. Animals recovered fully and were solidly immune to reinfection, in line with the view that treatment converts the otherwise virulent disease into a subclinical and immunizing infection.
机译：缺陷性干扰（DI）病毒RNA是由全长病毒RNA的主要缺失引起的，而这种缺失是在从头合成RNA的过程中自发发生的。这些RNA被包装成病毒颗粒，根据定义，该病毒颗粒是非感染性的，并被递送至病毒颗粒通常靶向的细胞。 DI RNA只能在共感染的传染性辅助病毒的帮助下复制，但是DI RNA的小尺寸使其比全长的全长复制品更多，因此该细胞可产生有缺陷的病毒体来代替传染性子代。与这种情况一致，通过施用DI病毒使甲型流感病毒-小鼠模型中的预期致死性疾病亚临床化，但动物对感染病毒产生了坚实的免疫力。因此，DI病毒被称为“干扰疫苗”。因为干扰疫苗在细胞内和分子水平上起作用，所以无论亚型如何，它都应有效对抗所有甲型流感病毒。在这里，我们使用了雪貂，它被广泛认为是人类流感的最佳模型。我们显示，一种具有H3N8病毒缺陷RNA的干扰疫苗几乎完全消除了由A / Sydney / 5/97（H3N2）引起的临床疾病，同时废除了发烧和临床症状的明显减少。动物完全康复并且对再感染具有牢固的免疫力，这与治疗将原本会致命的疾病转化为亚临床和免疫感染的观点一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号