A synthetic dengue virus antigen elicits enhanced antibody titers when linked to, but not mixed with, Mycobacterium tuberculosis HSP70 domain II

Kolli R; Khanam S; Jain M; Ganju L; Ram MS; Khanna N; Swaminathan S

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A synthetic dengue virus antigen elicits enhanced antibody titers when linked to, but not mixed with, Mycobacterium tuberculosis HSP70 domain II

机译：当与结核分枝杆菌HSP70结构域II连接但不混合时，合成的登革热病毒抗原引起增强的抗体效价

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Domain II of Mycobacterium tuberculosis (Mtb) heat shock protein 70 (HSP70), spanning amino acid residues 161-370, was covalently linked to a recently described synthetic dengue virus antigen to study the influence of the former on the immunogenicity of the latter. Using an enzyme-linked immunosorbent assay, dengue antigen-specific antibody titers elicited by the fusion protein in Balb/c mice were an order of magnitude higher than those elicited by either the synthetic dengue antigen alone or a physical mixture of the dengue antigen plus Mtb HSP70 domain II protein. Our data demonstrate that (i) Mtb HSP70 domain II is capable of potentiating B-cell response and (ii) it should be covalently linked to the target antigen to do so.

机译：结核分枝杆菌（Mtb）热休克蛋白70（HSP70）的结构域II，跨越氨基酸残基161-370，与最近描述的合成登革热病毒抗原共价连接，以研究前者对后者的免疫原性的影响。使用酶联免疫吸附试验，融合蛋白在Balb / c小鼠中引起的登革热抗原特异性抗体滴度比单独的合成登革热抗原或登革热抗原加Mtb的物理混合物引起的滴度高一个数量级。 HSP70域II蛋白。我们的数据表明（i）Mtb HSP70结构域II能够增强B细胞反应，并且（ii）应该与目标抗原共价连接才能达到此目的。

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《Vaccine》 |2006年第22期|共11页
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Kolli R; Khanam S; Jain M; Ganju L; Ram MS; Khanna N; Swaminathan S;
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中图分类卫生标准、卫生检查、医药管理;
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1. A synthetic dengue virus antigen elicits enhanced antibody titers when linked to, but not mixed with, Mycobacterium tuberculosis HSP70 domain II [J] . Kolli R, Khanam S, Jain M, Vaccine . 2006,第22期

机译：当与结核分枝杆菌HSP70结构域II连接但不混合时，合成的登革热病毒抗原引起增强的抗体效价
2. Induction of neutralizing antibodies specific to dengue virus serotypes 2 and 4 by a bivalent antigen composed of linked envelope domains III of these two serotypes. [J] . Khanam S, Etemad B, Khanna N, The American Journal of Tropical Medicine and Hygiene . 2006,第2期

机译：由由这两种血清型的连接的包膜结构域III组成的二价抗原诱导对登革热病毒血清型2和4具有特异性的中和抗体。
3. An Envelope Domain III–based Chimeric Antigen Produced in Pichia pastoris Elicits Neutralizing Antibodies Against All Four Dengue Virus Serotypes [J] . Behzad Etemad Gaurav Batra Rajendra Raut Satinder Dahiya Saima Khanam Sathyamangalam Swaminathan AND Navin Khanna* Am J Trop Med Hyg . 2008,第3期

机译：在毕赤酵母中产生的基于信封域III的嵌合抗原引发针对所有四种登革热病毒血清型的中和抗体。
4. EPITOPE AND AFFINITY DETERMINATION OF RECOMBINANT MYCOBACTERIUM TUBERCULOSIS AG85B ANTIGEN TOWARDS ANTI-AG85 ANTIBODIES USING PROTEOLYTIC-AFFINITY MASS SPECTROMETRY AND BIOSENSOR ANALYSIS [C] . Francesca Rinaldi, Loredana Lupu, Hendrik Rusche, International Mass Spectrometry Conference . 2018

机译：表位和亲和力测定结核分枝杆菌的重组分枝杆菌AG85B使用蛋白水解 - 亲和力质谱和生物传感器分析朝向抗AG85抗体的抗原
5. Antibody responses of horses to five major glycoproteins of equine herpesvirus 1 and specificity of responses to antigenic domains of glycoproteins B and C. [D] . Ostlund, Eileen Noel. 1992

机译：马对马疱疹病毒1的5种主要糖蛋白的抗体应答以及对糖蛋白B和C抗原域的应答的特异性。
6. Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies [O] . Lav Tripathi, Shailendra Mani, Rajendra Raut, 2020

机译：巴斯德毕赤酵母表达的登革热3包膜病毒样颗粒主要引起域III集中的高滴度中和抗体
7. Pichia pastoris-expressed Dengue 3 Envelope-based Virus-like Particles Elicit Predominantly Domain III-Focused High Titer Neutralizing Antibodies [O] . Lav eTripathi, Shailendra eMani, Rajendra eRaut, 2015

机译：pichia pastoris表达的登革热3包膜病毒样颗粒主要引发结构域III聚焦的高滴度中和抗体

A synthetic dengue virus antigen elicits enhanced antibody titers when linked to, but not mixed with, Mycobacterium tuberculosis HSP70 domain II

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