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首页> 外文期刊>Zygote >Developmental pattern of hexaploid mouse embryos produced by blastomere fusion of diploid and tetraploid embryos at the 2-cell stage
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Developmental pattern of hexaploid mouse embryos produced by blastomere fusion of diploid and tetraploid embryos at the 2-cell stage

机译：二倍体和四倍体胚胎的卵裂球融合在2细胞阶段产生的六倍体小鼠胚胎的发育模式

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摘要

Polyploid mouse embryos are important models for understanding the mechanisms of cleavage and preimplantation development in mammals. In this study, hexaploid (6n) mouse embryos were produced by the electrofusion of blastomeres from diploid (2n) and tetraploid (4n) embryos at the 2-cell stage. Furthermore, the developmental pattern of hexaploid embryos was evaluated by blastocyst rate, cell number, karyotype analysis, cytoskeleton staining and Oct-4 immunofluorescence. The results showed that 72.7% of the hexaploid embryos were able to develop to the blastocyst stage, which is a lower number than that found with normal diploid embryos (98.0%, p < 0.05). The cell number in hexaploid blastocyst was 12.3 +/- 2.0, which was less than that found in diploid or tetraploid blastocysts (41.2 +/- 7.2; 18.4 +/- 3.5). Karyotype analysis confirmed that the number of chromosomes in hexaploid embryos was 120. beta-Tubulin and Oct-4 immunofluorescence indicated that the hexaploid blastocysts were nearly lacking inner cell mass (ICM), but some blastomeres did show Oct-4-positive expression.
机译：多倍体小鼠胚胎是了解哺乳动物卵裂和植入前发育机制的重要模型。在这项研究中，六倍体（6n）小鼠胚胎是通过在2细胞阶段将二倍体（2n）和四倍体（4n）胚胎的卵裂球电融合而产生的。此外，通过胚泡率，细胞数量，核型分析，细胞骨架染色和Oct-4免疫荧光评估六倍体胚胎的发育模式。结果表明，有72.7％的六倍体胚胎能够发育到胚泡阶段，这一数量比正常二倍体胚胎的胚泡阶段低（98.0％，p <0.05）。六倍体胚泡中的细胞数为12.3 +/- 2.0，比二倍体或四倍体胚泡中的细胞数少（41.2 +/- 7.2; 18.4 +/- 3.5）。染色体核型分析证实六倍体胚胎中的染色体数目为120。β-微管蛋白和Oct-4免疫荧光表明六倍体胚泡几乎没有内部细胞团（ICM），但某些卵裂球确实显示了Oct-4阳性表达。

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