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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Does the metal influence non-covalent binding of complexes to DNA?
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Does the metal influence non-covalent binding of complexes to DNA?

机译：金属会影响复合物与DNA的非共价结合吗？

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摘要

Electrospray ionisation mass spectrometry, absorption spectrophotometry and circular dichroism spectroscopy were used to investigate the binding of a series of nickel complexes with the general formula [Ni(phen)(2)L](2+) (L = phen, dpq, dpqC and dppz) to a double stranded DNA hexadecamer. In addition, the binding of the complexes to pUC9 negatively supercoiled plasmid DNA was examined using gel electrophoresis, and their ability to inhibit DNA transcription was measured. Each of the above techniques showed that DNA binding strengthened as the size of the unique ligand L was increased. Comparison of the above results with those obtained previously, and presented here for the first time for the analogous series of ruthenium complexes [ Ru(phen)(2)L](2+), showed that changing the metal ion from nickel to ruthenium consistently resulted in significant increases in DNA binding affinity.
机译：电喷雾电离质谱，吸收分光光度法和圆二色光谱用于研究一系列通式为[Ni（phen）（2）L]（2+）的镍配合物的结合（L = phen，dpq，dpqC和dppz）连接到双链DNA hexadecamer。另外，使用凝胶电泳检查复合物与pUC9负超螺旋质粒DNA的结合，并测量其抑制DNA转录的能力。上述每种技术均显示，随着独特配体L尺寸的增加，DNA结合增强。将上述结果与先前获得的结果进行比较，并首次在此处首次提出类似系列的钌配合物[Ru（phen）（2）L]（2+），表明金属离子始终如一地从镍变为钌导致DNA结合亲和力显着增加。

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