...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Iron-binding properties of plant phenolics and cranberry's bio-effects
【24h】

Iron-binding properties of plant phenolics and cranberry's bio-effects

机译：植物酚类物质的铁结合特性和酸果蔓的生物效应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The health benefits of cranberries have long been recognized. However, the mechanisms behind its function are poorly understood. We have investigated the iron-binding properties of quercetin, the major phenolic phytochemical present in cranberries, and other selected phenolic compounds ( chrysin, 3-hydroxyflavone, 3 ',4 '-dihydroxy flavone, rutin, and flavone) in aqueous media using UV/vis, NMR and EPR spectroscopies and ESI-Mass spectrometry. Strong iron-binding properties have been confirmed for the compounds containing the "iron-binding motifs" identified in their structures. The apparent binding constants are estimated to be in the range of 10(6) M-1 to 10(12) M-2 in phosphate buffer at pH 7.2. Surprisingly, quercetin binds Fe2+ even stronger than the well known Fe2+-chelator ferrozine at pH 7.2. This may be the first example of an oxygen-based ligand displaying stronger Fe2+-binding affinity than a strong nitrogen-based Fe2+-chelator. The strong Fe-binding properties of these phenolics argue that they may be effective in modulating cellular iron homeostasis under physiological conditions. Quercetin can completely suppress Fenton chemistry both at micromolar levels and in the presence of major cellular iron chelators like ATP or citrate. However, the radical scavenging activity of quercetin provides only partial protection against Fenton chemistry-mediated damage while Fe chelation by quercetin can completely inhibit Fenton chemistry, indicating that the chelation may be key to its antioxidant activity. These results demonstrate that quercetin and other phenolic compounds can effectively modulate iron biochemistry under physiologically relevant conditions, providing insight into the mechanism of action of bio-active phenolics.
机译：蔓越莓的健康益处早已为人所公认。但是，对其功能背后的机制了解甚少。我们研究了槲皮素，蔓越莓中主要的酚类植物化学成分以及其他选定的酚类化合物（菊花蛋白，3-羟基黄酮，3'，4'-二羟基黄酮，芦丁和黄酮）在水中的铁结合特性。 / vis，NMR和EPR光谱以及ESI-质谱。对于在其结构中鉴定出的含有“铁结合基序”的化合物，已证实具有强的铁结合性能。在pH 7.2的磷酸盐缓冲液中，表观结合常数估计为10（6）M-1至10（12）M-2。出人意料的是，槲皮素在pH 7.2时与Fe2 +的结合甚至比众所周知的Fe2 +-螯合剂的二嗪铁更牢固。这可能是基于氧的配体显示出比基于氮的强Fe2 +螯合剂更强的Fe2 +结合亲和力的第一个例子。这些酚类化合物具有很强的铁结合特性，说明它们在生理条件下可能有效调节细胞铁稳态。槲皮素可以在微摩尔水平和存在主要细胞铁螯合剂（如ATP或柠檬酸盐）的情况下完全抑制Fenton化学。然而，槲皮素的自由基清除活性仅提供了针对Fenton化学介导的损害的部分保护，而槲皮素的Fe螯合可以完全抑制Fenton化学，表明该螯合可能是其抗氧化活性的关键。这些结果表明，槲皮素和其他酚类化合物可以在生理相关条件下有效调节铁的生物化学，从而深入了解生物活性酚类的作用机理。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号