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Limited predictive value of blastomere angle of division in trophectoderm and inner cell mass specification

机译：滋养层和内部细胞质量指标中卵裂球分裂角的有限预测值

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The formation of trophectoderm (TE) and pluripotent inner cell mass (ICM) is one of the earliest events duringmammalianembryogenesis. It is believed that the orientation of division of polarised blastomeres in the 8- and 16-cell stage embryo determines the fate of daughter cells, based on how asymmetrically distributed lineage determinants are segregated. To investigate the relationship between angle of division and subsequent fate in unperturbed embryos, we constructed cellular resolution digital representations of the development ofmouse embryos from the morula to early blastocyst stage, based on 4D confocal image volumes. We find that at the 16-cell stage, very few inside cells are initially produced as a result of cell division, but that the number increases due to cellmovement.Contrary to expectations, outside cells at the 16-cell stage represent a heterogeneous population, with some fated to contributing exclusively to the TE and others capable of contributing to both the TE and ICM. Our data support the view that factors other than the angle of division, such as the position of a blastomere, play a major role in the specification of TE and ICM.
机译：滋养外胚层（TE）和多能内细胞团（ICM）的形成是哺乳动物胚胎发生过程中最早的事件之一。据信，基于不对称分布的谱系决定簇的分离方式，极化卵裂球在8和16细胞期胚胎中分裂的方向决定了子代细胞的命运。为了研究不受干扰的胚胎的分裂角度与后续命运之间的关系，我们基于4D共聚焦图像体积构建了从桑ula胚到胚泡早期的小鼠胚胎发育的细胞分辨率数字表示。我们发现，在16个细胞阶段，最初由于细胞分裂而产生的内部细胞很少，但是由于细胞运动而增加了数量。与预期相反，在16个细胞阶段的外部细胞代表了异质群体，有些注定要专门为TE做出贡献，而另一些注定要为TE和ICM做出贡献。我们的数据支持这样一种观点，即除分裂角以外的其他因素（例如卵裂球的位置）在TE和ICM规范中起着重要作用。

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