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首页> 外文期刊>Development >Translational repression of a C. elegans Notch mRNA by the STAR/KH domain protein GLD-1.
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Translational repression of a C. elegans Notch mRNA by the STAR/KH domain protein GLD-1.

机译：STAR / KH域蛋白GLD-1对秀丽隐杆线虫Notch mRNA的翻译抑制。

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摘要

In C. elegans, the Notch receptor GLP-1 is localized within the germline and early embryo by translational control of glp-1 mRNA. RNA elements in the glp-1 3'untranslated region (3' UTR) are necessary for repression of glp-1 translation in germ cells, and for localization of translation to anterior cells of the early embryo. The direct regulators of glp-1 mRNA are not known. Here, we show that a 34 nucleotide region of the glp-1 3' UTR contains two regulatory elements, an element that represses translation in germ cells and posterior cells of the early embryo, and an element that inhibits repressor activity to promote translation in the embryo. Furthermore, we show that the STAR/KH domain protein GLD-1 binds directly and specifically to the repressor element. Depletion of GLD-1 activity by RNA interference causes loss of endogenous glp-1 mRNA repression in early meiotic germ cells, and in posterior cells of the early embryo. Therefore, GLD-1 is a direct repressor of glp-1 translation at two developmental stages. These results suggest a new function for GLD-1 in regulating early embryonic asymmetry. Furthermore, these observations indicate that precise control of GLD-1 activity by other regulatory factors is important to localize this Notch receptor, and contributes to the spatial organization of Notch signaling.
机译：在秀丽隐杆线虫中，Notch受体GLP-1通过glp-1 mRNA的翻译控制定位在种系和早期胚胎中。 glp-1 3'非翻译区（3'UTR）中的RNA元件对于抑制生殖细胞中glp-1的翻译以及将翻译定位到早期胚胎的前壁细胞是必需的。 glp-1 mRNA的直接调节剂是未知的。在这里，我们显示glp-1 3'UTR的34个核苷酸区域包含两个调控元件，一个抑制早期胚胎的生殖细胞和后代细胞翻译的元件，一个抑制阻遏物活性以促进翻译的元件。胚胎。此外，我们表明STAR / KH域蛋白GLD-1直接和特异性结合阻遏物。 RNA干扰对GLD-1活性的破坏会导致减数分裂早期生殖细胞和早期胚胎后代细胞内源性glp-1 mRNA阻遏的丧失。因此，GLD-1在两个发育阶段是glp-1翻译的直接阻遏物。这些结果表明GLD-1在调节早期胚胎不对称性中的新功能。此外，这些观察结果表明，其他调节因子对GLD-1活性的精确控制对于定位该Notch受体很重要，并有助于Notch信号的空间组织。

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