...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Development >Arabidopsis MSI1 is required for epigenetic maintenance of reproductive development.
【24h】

Arabidopsis MSI1 is required for epigenetic maintenance of reproductive development.

机译：拟南芥MSI1是生殖发育的表观遗传维持所必需的。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

WD40 repeat proteins similar to yeast MSI1 are conserved in animals and plants, in which they participate in complexes involved in chromatin metabolism. Although MSI1-like proteins are well characterised biochemically, their function in the development of multicellular eukaryotes is not well understood. We constructed Arabidopsis plants in which the AtMSI1 protein level was altered. Strong ectopic expression of AtMSI1 produced no visible altered phenotype, but reduction of AtMSI1 dramatically affected development. The primary shoot apical meristem was unable to develop organs after the transition to flowering. Flowers that developed on floral shoots from axillary meristems experienced a progressive loss of floral morphology, including a reduction in size of the petals and stamens and the development of carpel-like sepals. Ovule development was disrupted in all flowers, resulting in complete female sterility. Molecular analysis of the mutant plants revealed that AtMSI1 is required to maintain the correct temporal and organ-specific expression of homeotic genes, including AGAMOUS and APETALA2. In contrast, FAS1 and FAS2, which together with AtMSI1 form the chromatin assembly complex CAF-1, are not required for repression of these genes. Therefore, AtMSI1 has specific functions in addition to CAF-1-mediated chromatin assembly. Efficient formation of heterochromatin, but not methylation of centromeric DNA repeats, depends on AtMSI1 presence demonstrating a key role of AtMSI1 in maintenance of chromatin structure.
机译：与酵母MSI1类似的WD40重复蛋白在动植物中是保守的，它们参与染色质代谢相关的复合物。尽管MSI1样蛋白的生化特性已得到很好的表征，但它们在多细胞真核生物发育中的功能尚不十分清楚。我们构建了AtMSI1蛋白水平发生变化的拟南芥植物。 AtMSI1的强烈异位表达没有产生明显的表型改变，但是AtMSI1的减少显着影响了发育。过渡到开花后，初生的茎尖分生组织无法发育器官。从腋生分生组织的芽上发育的花经历了逐渐失去的花形，包括花瓣和雄蕊的大小减少以及心皮状萼片的发育。所有花的胚珠发育被破坏，导致完全雌性不育。突变植物的分子分析显示，需要AtMSI1来维持同源基因（包括AGAMOUS和APETALA2）的正确的时间和器官特异性表达。相反，FAS1和FAS2与AtMSI1一起形成染色质组装复合物CAF-1，则不需要这些基因。因此，除了CAF-1介导的染色质组装，AtMSI1还具有特定的功能。异染色质的有效形成而不是着丝粒DNA重复序列的甲基化取决于AtMSI1的存在，这证明了AtMSI1在维持染色质结构中的关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号