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首页> 外文期刊>Development >A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration.
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A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration.

机译：脊椎动物无交叉静脉2同源物调节BMP活性和神经c细胞迁移。

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Previous work has revealed that proteins that bind to bone morphogenetic proteins (BMPs) and inhibit their signalling have a crucial role in the spatial and temporal regulation of cell differentiation and cell migration by BMPs. We have identified a chick homologue of crossveinless 2, a Drosophila gene that was identified in genetic studies as a promoter of BMP-like signalling. Chick Cv-2 has a conserved structure of five cysteine-rich repeats similar to those found in several BMP antagonists, and a C-terminal Von Willebrand type D domain. Cv-2 is expressed in the chick embryo in a number of tissues at sites at which elevated BMP signalling is required. One such site of expression is premigratory neural crest, in which at trunk levels threshold levels of BMP activity are required to initiate cell migration. We show that, when overexpressed, Cv-2 can weakly antagonise BMP4 activity in Xenopus embryos, but that in other in vitro assays Cv-2 can increase the activity of co-expressed BMP4. Furthermore, we find that increased expression of Cv-2 causes premature onset of trunk neural crest cell migration in the chick embryo, indicative of Cv-2 acting to promote BMP activity at an endogenous site of expression. We therefore propose that BMP signalling is modulated both by antagonists and by Cv-2 that acts to elevate BMP activity.
机译：先前的工作表明，与骨形态发生蛋白（BMP）结合并抑制其信号传导的蛋白在BMPs对细胞分化和细胞迁移的时空调节中起着至关重要的作用。我们已经确定了crossveinless 2，果蝇基因，在基因研究中被确定为BMP样信号的启动子的雏鸡同源物。小鸡Cv-2具有5个富含半胱氨酸的重复序列的保守结构，类似于在几种BMP拮抗剂中发现的重复序列，以及一个C端Von Willebrand D型结构域。 Cv-2在需要提高BMP信号传导的位点的许多组织中的雏鸡胚胎中表达。这种表达位点之一是迁移前的神经rest，其中在躯干水平，需要阈值水平的BMP活性来启动细胞迁移。我们显示，当Cv-2过表达时，它可以弱化非洲爪蟾胚胎中的BMP4活性，但是在其他体外测定中，Cv-2可以增加共表达的BMP4的活性。此外，我们发现Cv-2的表达增加会引起雏鸡胚胎干神经c细胞迁移的过早发作，这表明Cv-2在内源性表达位点发挥促进BMP活性的作用。因此，我们提出BMP信号传导既受拮抗剂又受Cv-2的调节，而Cv-2的作用是提高BMP的活性。

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