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首页> 外文期刊>Development >Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.
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Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.

机译：精氨酸甲基转移酶胶囊对于果蝇中剪接体Sm蛋白的甲基化和生殖细胞的形成至关重要。

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摘要

Although arginine modification has been implicated in a number of cellular processes, the in vivo requirement of protein arginine methyltransferases (PRMTs) in specific biological processes remain to be clarified. In this study we characterize the Drosophila PRMT Capsuleen, homologous to human PRMT5. During Drosophila oogenesis, catalytic activity of Capsuleen is necessary for both the assembly of the nuage surrounding nurse cell nuclei and the formation of the pole plasm at the posterior end of the oocyte. In particular, we show that the nuage and pole plasm localization of Tudor, an essential component for germ cell formation, are abolished in csul mutant germ cells. We identify the spliceosomal Sm proteins as in vivo substrates of Capsuleen and demonstrate that Capsuleen, together with its associated protein Valois, is essential for the synthesis of symmetric di-methylated arginyl residues in Sm proteins. Finally, we show that Tudor can be targeted to the nuage in the absence of Sm methylation by Capsuleen, indicating that Tudor localization and Sm methylation are separate processes. Our results thus reveal the role of a PRMT in protein localization in germ cells.
机译：尽管精氨酸修饰已涉及许多细胞过程，但在特定生物学过程中蛋白质精氨酸甲基转移酶（PRMT）的体内需求仍有待阐明。在这项研究中，我们表征了果蝇PRMT胶囊，与人类PRMT5同源。在果蝇卵子发生过程中，胶囊的催化活性对于护士细胞核周围的小核的组装和卵母细胞后端极质的形成都是必需的。特别是，我们显示出csul突变型生殖细胞中都没有了Tudor（生殖细胞形成的基本组成部分）的位点和极质定位。我们确定剪接的Sm蛋白为Capsuleen的体内底物，并证明Capsuleen及其相关蛋白Valois，对于Sm蛋白中对称的二甲基化精氨酰基残基的合成至关重要。最后，我们表明，在没有Capsuleen的Sm甲基化的情况下，Tudor可以靶向到Nuage，这表明Tudor定位和Sm甲基化是独立的过程。因此，我们的结果揭示了PRMT在生殖细胞蛋白质定位中的作用。

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