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首页> 外文期刊>Development >RhoGEF2 and the formin Dia control the formation of the furrow canal by directed actin assembly during Drosophila cellularisation.
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RhoGEF2 and the formin Dia control the formation of the furrow canal by directed actin assembly during Drosophila cellularisation.

机译：在果蝇细胞化过程中，RhoGEF2和福尔马Dia通过定向肌动蛋白组装控制沟渠的形成。

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摘要

The physical interaction of the plasma membrane with the associated cortical cytoskeleton is important in many morphogenetic processes during development. At the end of the syncytial blastoderm of Drosophila the plasma membrane begins to fold in and forms the furrow canals in a regular hexagonal pattern. Every furrow canal leads the invagination of membrane between adjacent nuclei. Concomitantly with furrow canal formation, actin filaments are assembled at the furrow canal. It is not known how the regular pattern of membrane invagination and the morphology of the furrow canal is determined and whether actin filaments are important for furrow canal formation. We show that both the guanyl-nucleotide exchange factor RhoGEF2 and the formin Diaphanous (Dia) are required for furrow canal formation. In embryos from RhoGEF2 or dia germline clones, furrow canals do not form at all or are considerably enlarged and contain cytoplasmic blebs. Both Dia and RhoGEF2 proteins are localised at the invagination site prior to formation of the furrow canal. Whereas they localise independently of F-actin, Dia localisation requires RhoGEF2. The amount of F-actin at the furrow canal is reduced in dia and RhoGEF2 mutants, suggesting that RhoGEF2 and Dia are necessary for the correct assembly of actin filaments at the forming furrow canal. Biochemical analysis shows that Rho1 interacts with both RhoGEF2 and Dia, and that Dia nucleates actin filaments. Our results support a model in which RhoGEF2 and dia control position, shape and stability of the forming furrow canal by spatially restricted assembly of actin filaments required for the proper infolding of the plasma membrane.
机译：在发育过程中的许多形态发生过程中，质膜与相关的皮质细胞骨架的物理相互作用是重要的。在果蝇合胞体胚盘的末尾，质膜开始折叠并以规则的六边形形式形成沟渠。每条沟渠都导致相邻核之间的膜内陷。伴随着沟管的形成，肌动蛋白丝在沟管处组装。尚不清楚如何确定膜内陷的规则模式和沟管的形态以及肌动蛋白丝对于沟管的形成是否重要。我们表明鸟嘌呤-核苷酸交换因子RhoGEF2和福尔明Diaphanous（Dia）是沟渠形成所需的。在来自RhoGEF2或dia种系克隆的胚胎中，沟渠根本不形成或没有明显扩大，并含有胞质泡。在形成沟渠之前，Dia和RhoGEF2蛋白都位于内陷位点。尽管它们独立于F-肌动蛋白定位，但Dia定位需要RhoGEF2。在dia和RhoGEF2突变体中，沟管中F-肌动蛋白的量减少，这表明RhoGEF2和Dia对于在形成的沟管中正确组装肌动蛋白丝是必需的。生化分析表明Rho1与RhoGEF2和Dia都相互作用，并且Dia会成核肌动蛋白丝。我们的结果支持了一个模型，其中RhoGEF2和dia通过空间限制肌动蛋白丝的适当折叠所需的肌动蛋白丝组装来控制沟沟的位置，形状和稳定性。

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