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首页> 外文期刊>Development >Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events.
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Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events.

机译：Bchs，一个BEACH域蛋白，在突触形态发生和其他发育事件中拮抗Rab11。

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摘要

BEACH proteins, an evolutionarily conserved family characterized by the presence of a BEACH (Beige and Chediak-Higashi) domain, have been implicated in membrane trafficking, but how they interact with the membrane trafficking machinery is unknown. Here we show that the Drosophila BEACH protein Bchs (Blue cheese) acts during development as an antagonist of Rab11, a small GTPase involved in vesicle trafficking. We find that reduction in, or loss of, bchs function restores viability and normal bristle development in animals with reduced rab11 function, while reductions in rab11 function exacerbate defects caused by bchs overexpression in the eye. Consistent with a role for Bchs in modulating Rab11-dependent trafficking, Bchs protein is associated with vesicles and extensively colocalized with Rab11 at the neuromuscular junction (NMJ). At the NMJ, we find that rab11 is important for synaptic morphogenesis, as reductions in rab11 function cause increases in bouton density and branching. These defects are also suppressed by loss of bchs. Taken together, these data identify Bchs as an antagonist of Rab11 during development and uncover a role for these regulators of vesicle trafficking in synaptic morphogenesis. This raises the interesting possibility that Bchs and other BEACH proteins may regulate vesicle traffic via interactions with Rab GTPases.
机译：BEACH蛋白是一个进化保守的家族，其特征是存在BEACH（Beige和Chediak-Higashi）域，该蛋白与膜运输有关，但是它们如何与膜运输机制相互作用尚不清楚。在这里，我们显示果蝇BEACH蛋白Bchs（蓝纹奶酪）在开发过程中充当Rab11（一种参与小泡运输的小型GTPase）的拮抗剂。我们发现，bchs功能的降低或丧失可恢复rab11功能降低的动物的活力和正常的鬃毛发育，而rab11功能的降低会加剧由眼睛中bchs过表达引起的缺陷。与Bchs在调节Rab11依赖性运输中的作用一致，Bchs蛋白与囊泡相关，并在神经肌肉接头（NMJ）处与Rab11广泛共定位。在NMJ，我们发现rab11对于突触形态发生很重要，因为rab11功能的降低会导致钮扣密度和分支增加。这些缺陷也可以通过bch的丢失来抑制。综上所述，这些数据确定了Bchs在发育过程中是Rab11的拮抗剂，并揭示了这些小泡运输的调节剂在突触形态发生中的作用。这引起了有趣的可能性，即Bchs和其他BEACH蛋白可能通过与Rab GTPases的相互作用调节囊泡运输。

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