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首页> 外文期刊>Development >The RhoGEF Pebble is required for cell shape changes during cell migration triggered by the Drosophila FGF receptor Heartless.
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The RhoGEF Pebble is required for cell shape changes during cell migration triggered by the Drosophila FGF receptor Heartless.

机译：果蝇FGF受体Heartless触发的细胞迁移过程中，细胞形状变化需要RhoGEF Pebble。

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摘要

The FGF receptor Heartless (HTL) is required for mesodermal cell migration in the Drosophila gastrula. We show that mesoderm cells undergo different phases of specific cell shape changes during mesoderm migration. During the migratory phase, the cells adhere to the basal surface of the ectoderm and exhibit extensive protrusive activity. HTL is required for the protrusive activity of the mesoderm cells. Moreover, the early phenotype of htl mutants suggests that HTL is required for the adhesion of mesoderm cells to the ectoderm. In a genetic screen we identified pebble (pbl) as a novel gene required for mesoderm migration. pbl encodes a guanyl nucleotide exchange factor (GEF) for RHO1 and is known as an essential regulator of cytokinesis. We show that the function of PBL in cell migration is independent of the function of PBL in cytokinesis. Although RHO1 acts as a substrate for PBL in cytokinesis, compromising RHO1 function in the mesoderm does not block cell migration. These data suggest that the function of PBL in cell migration might be mediated through a pathway distinct from RHO1. This idea is supported by allele-specific differences in the expressivity of the cytokinesis and cell migration phenotypes of different pbl mutants. We show that PBL is autonomously required in the mesoderm for cell migration. Like HTL, PBL is required for early cell shape changes during mesoderm migration. Expression of a constitutively active form of HTL is unable to rescue the early cellular defects in pbl mutants, suggesting that PBL is required for the ability of HTL to trigger these cell shape changes. These results provide evidence for a novel function of the Rho-GEF PBL in HTL-dependent mesodermal cell migration.
机译：FGF受体无心（HTL）是果蝇腹中中胚层细胞迁移所必需的。我们显示中胚层细胞在中胚层迁移过程中经历特定细胞形状变化的不同阶段。在迁移阶段，细胞粘附于外胚层的基底表面并表现出广泛的突出活性。 HTL是中胚层细胞突出活动所必需的。此外，htl突变体的早期表型表明，HTL是中胚层细胞与外胚层粘附的必需条件。在遗传筛选中，我们确定了卵石（pbl）是中胚层迁移所需的新基因。 pbl编码RHO1的鸟苷酸核苷酸交换因子（GEF），被称为胞质分裂的重要调节剂。我们表明，PBL在细胞迁移中的功能独立于PBL在胞质分裂中的功能。尽管RHO1在胞质分裂中充当PBL的底物，但损害中胚层中RHO1的功能不会阻止细胞迁移。这些数据表明，PBL在细胞迁移中的功能可能是通过不同于RHO1的途径介导的。不同pbl突变体的胞质分裂表达和细胞迁移表型的等位基因特异性差异支持了该想法。我们表明，PBL是中胚层中自主需要的细胞迁移。与HTL一样，中胚层迁移期间早期细胞形状变化也需要PBL。 HTL的组成型活性形式的表达不能挽救pbl突变体中早期的细胞缺陷，这表明HTL触发这些细胞形状变化的能力需要PBL。这些结果提供了Rho-GEF PBL在HTL依赖的中胚层细胞迁移中的新功能的证据。

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