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首页> 外文期刊>Development >Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.
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Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.

机译：海藻胚胎中转录因子和信号分子的基因表达谱：对基因网络的全面理解。

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Achieving a real understanding of animal development obviously requires a comprehensive rather than partial identification of the genes working in each developmental process. Recent decoding of genome sequences will enable us to perform such studies. An ascidian, Ciona intestinalis, one of the animals whose genome has been sequenced, is a chordate sharing a basic body plan with vertebrates, although its genome contains less paralogs than are usually seen in vertebrates. In the present study, we discuss the genomewide approach to networks of developmental genes in Ciona embryos. We focus on transcription factor genes and some major groups of signal transduction genes. These genes are comprehensively listed and examined with regard to their embryonic expression by in situ hybridization (http://ghost.zool.kyoto-u.ac.jp/tfst.html). The results revealed that 74% of the transcription factor genes are expressed maternally and that 56% of the genes are zygotically expressed during embryogenesis. Of these, 34% of the transcription factor genes are expressed both maternally and zygotically. The number of zygotically expressed transcription factor genes increases gradually during embryogenesis. As an example, and taking advantage of this comprehensive description of gene expression profiles, we identified transcription factor genes and signal transduction genes that are expressed at the early gastrula stage and that work downstream of beta-catenin, FoxD and/or Fgf9/16/20. Because these three genes are essential for ascidian endomesoderm specification, transcription factor genes and signal transduction genes involved in each of the downstream processes can be deduced comprehensively using the present approach.
机译：要真正了解动物的发育，显然需要对每个发育过程中起作用的基因进行全面而非部分的鉴定。基因组序列的最新解码将使我们能够进行此类研究。一种海鞘小肠Ciona intestinalis，是一种基因组已被测序的动物，是一种与脊椎动物共有基本身体计划的碳酸盐，尽管其基因组所含的旁系同源物比通常在脊椎动物中所见的少。在本研究中，我们讨论了Ciona胚胎发育基因网络的全基因组方法。我们专注于转录因子基因和信号转导基因的一些主要类别。通过原位杂交全面列出了这些基因并对其胚胎表达进行了检查（http://ghost.zool.kyoto-u.ac.jp/tfst.html）。结果表明，74％的转录因子基因在母体中表达，56％的基因在胚发生过程中合子表达。其中，有34％的转录因子基因在母体和合子中表达。自发表达的转录因子基因的数量在胚胎发生过程中逐渐增加。例如，并利用对基因表达谱的全面描述，我们鉴定了在早胃期表达并在β-catenin，FoxD和/或Fgf9 / 16 /下游起作用的转录因子基因和信号转导基因。 20因为这三个基因对于海鞘内膜规范是必不可少的，所以可以使用本方法全面推导涉及每个下游过程的转录因子基因和信号转导基因。

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