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首页> 外文期刊>Development >GSK3 is a multifunctional regulator of Dictyostelium development.
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GSK3 is a multifunctional regulator of Dictyostelium development.

机译：GSK3是双歧杆菌发育的多功能调节剂。

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Glycogen synthase kinase 3 (GSK3) is a central regulator of metazoan development and the Dictyostelium GSK3 homologue, GskA, also controls cellular differentiation. The originally derived gskA-null mutant exhibits a severe pattern formation defect. It forms very large numbers of pre-basal disc cells at the expense of the prespore population. This defect arises early during multicellular development, making it impossible to examine later functions of GskA. We report the analysis of a gskA-null mutant, generated in a different parental strain, that proceeds through development to form mature fruiting bodies. In this strain, Ax2/gskA-, early development is accelerated and slug migration greatly curtailed. In a monolayer assay of stalk cell formation, the Ax2/gskA- strain is hypersensitive to the stalk cell-inducing action of DIF-1 but largely refractory to the repressive effect exerted by extracellular cAMP. During normal development, apically situated prestalk cells express the ecmB gene just as they commit themselves to stalk cell differentiation. In the Ax2/gskA- mutant, ecmB is expressed throughout the prestalk region of the slug, suggesting that GskA forms part of the repressive signalling pathway that prevents premature commitment to stalk cell differentiation. GskA may also play an inductive developmental role, because microarray analysis identifies a large gene family, the 2C family, that require gskA for optimal expression. These observations show that GskA functions throughout Dictyostelium development, to regulate several key aspects of cellular patterning.
机译：糖原合酶激酶3（GSK3）是后生动物发育的主要调节剂，而硬脂酸单胞菌GSK3同源物GskA也控制细胞分化。最初衍生的gskA-null突变体表现出严重的模式形成缺陷。它以孢子的数量为代价形成了非常大量的前基底盘细胞。此缺陷在多细胞发育的早期出现，使得无法检查GskA的后续功能。我们报告了在不同的亲本菌株中产生的gskA-null突变体的分析，该突变体通过发育形成成熟的子实体。在此菌株Ax2 / gskA-中，早期发育得到加速，而团状菌的迁移大大减少。在茎细胞形成的单层测定中，Ax2 / gskA-菌株对DIF-1的茎细胞诱导作用高度敏感，但对细胞外cAMP产生的阻抑作用却很难。在正常发育过程中，顶端定位的前茎细胞表达ecmB基因，就像它们致力于茎细胞分化一样。在Ax2 / gskA-突变体中，ecmB在子弹的前茎区域均表达，这表明GskA形成了抑制信号转导途径的一部分，阻止了过早地致力于茎细胞分化。 GskA也可能发挥诱导性作用，因为微阵列分析确定了需要gskA才能获得最佳表达的大基因家族2C家族。这些观察结果表明，GskA在整个网盘菌的发育过程中发挥功能，以调节细胞模式的几个关键方面。

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