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首页> 外文期刊>Development Genes and Evolution >Identification and developmental expression of leucine-rich repeat-containing G protein-coupled receptor 6 (lgr6) in the medaka fish, Oryzias latipes
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Identification and developmental expression of leucine-rich repeat-containing G protein-coupled receptor 6 (lgr6) in the medaka fish, Oryzias latipes

机译：鉴定和发展表达富含亮氨酸的重复序列的G蛋白偶联受体6（lgr6）在in鱼中的表达。

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摘要

G protein-coupled receptors are critical regulators of diverse developmental processes such as oocyte maturation, fertilization, gastrulation, and organogenesis. To further study the molecular mechanisms underlying these processes, we cloned and characterized the orphan leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6), a stem cell marker in mammalian hair follicles, in medaka fish, Oryzias latipes. To examine the expression pattern of lgr6, we performed whole-mount in situ hybridization (WISH) during embryogenesis. The expression of lgr6 was first detected as a band in the anterior part of the posterior brain vesicle in 0.5-1 day post fertilization (dpf) embryos. This band disappeared by 2 dpf, but new signals appeared in the otic vesicles bordering the original band and also detected in the nasal placode and posterior lateral line primordia. At later stages (3-5 dpf), lgr6 was widely expressed in the brain, otic vesicle, neuromasts, root of the pectoral fin, cranial cartilage, and gut. Then, we conducted more detailed expression analysis of lgr6 in adult gut using WISH and immunohistochemical staining. Lgr6-positive cells were detected in the crypt-like proliferative zone and in parts of the villus. We also performed RT-PCR of mRNAs from different tissues. The lgr6 mRNA was found highest in the kidney and gill. The transcript was also present in the brain, heart, liver, spleen, intestine, skeletal muscle, testis, and ovary, similar to that of mammalian LGR6. These results suggest that medaka lgr6 plays an important role in organ development during embryogenesis and serves as a good molecular marker for future studies of postembryonic organ-specific development in mammals.
机译：G蛋白偶联受体是诸如卵母细胞成熟，受精，胃形成和器官发生等多种发育过程的关键调节剂。为了进一步研究这些过程的分子机制，我们克隆并鉴定了孤立的富含孤亮氨酸重复序列的G蛋白偶联受体6（LGR6），该蛋白是哺乳动物毛囊中鱼Oryzias latipes的干细胞标记。为了检查lgr6的表达模式，我们在胚胎发生过程中进行了完整的原位杂交（WISH）。在受精（dpf）胚胎后0.5-1天，首先在脑后囊泡前部的一条带中检测到lgr6的表达。该条带消失了2 dpf，但新信号出现在与原始条带接壤的耳囊中，并且在鼻斑和后外侧原基中也检测到。在后期（3-5 dpf），lgr6在脑，耳囊，神经瘤，胸鳍根，颅软骨和肠中广泛表达。然后，我们使用WISH和免疫组化染色对成年肠道中的lgr6进行了更详细的表达分析。在隐窝样增生区和部分绒毛中检测到Lgr6阳性细胞。我们还对来自不同组织的mRNA进行了RT-PCR。发现lgr6 mRNA在肾脏和6中最高。转录物也存在于脑，心脏，肝脏，脾脏，肠，骨骼肌，睾丸和卵巢中，与哺乳动物的LGR6类似。这些结果表明，medaka lgr6在胚胎发生期间的器官发育中起着重要作用，并作为哺乳动物胚胎后器官特异性发育的未来研究的良好分子标记。

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