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首页> 外文期刊>Development Genes and Evolution >Vasa and nanos are coexpressed in somatic and germ line tissue from early embryonic cleavage stages through adulthood in the polychaete Capitella sp. I.
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Vasa and nanos are coexpressed in somatic and germ line tissue from early embryonic cleavage stages through adulthood in the polychaete Capitella sp. I.

机译：Vasa和nanos在早期的卵裂期至成年期的多毛Cap Capitella sp中，在体细胞和种系组织中共表达。一世。

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摘要

Members of the vasa and nanos gene families are involved in germ line development in a number of diverse animals. As a polychaete annelid model for studies of the germ line, Capitella sp. I has several advantages including the presence of dedicated gonads, individuals that reproduce multiple times, and the presence of males, females, and hermaphrodites. Germ line development has not been characterized in Capitella sp. I, nor is the mechanism of germ line specification generally well understood in annelids. We have cloned vasa and nanos orthologues from Capitella sp. I and found that both CapI-vasa and CapI-nanos transcripts are expressed in developing gametes of sexually mature adults. Characterization of both these genes during embryonic, larval, and juveniles stages reveals expression in multiple somatic tissues for CapI-vasa and CapI-nanos with largely overlapping but not identical expression patterns. In early cleavage stages, both transcripts are broadly expressed; following gastrulation, expression is observed in the presumptive brain, mesodermal bands, and developing foregut. Using CapI-nanos and CapI-vasa as markers, we have identified putative primordial germ cells (PGCs) in larvae, which are initially present as small bilateral clusters in segment 4 and as a single cluster at late larval stages. In adults, a single large cluster of putative PGCs is present in segments 5 and 6. In addition to highlighting differences in expression profiles for these two genes among lophotrochozoans, we present a hypothesis concerning the origin and development of PGCs in Capitella sp. I.
机译：瓦萨和纳米基因家族的成员参与了许多不同动物的种系发展。作为研究生殖细胞系的多壳类无脊椎动物模型，Capitella sp.。我有几个优点，包括专用性腺，多次繁殖的个体以及雄性，雌性和雌雄同体的存在。 Capitella sp。没有鉴定种系的发育。我，种系规范的机制也没有在花粉中得到很好的理解。我们已经从Capitella sp。克隆了vasa和nanos直向同源物。我发现CapI-vasa和CapI-nanos转录本均在性成熟成年人的配子中表达。这两个基因在胚胎，幼虫和幼虫阶段的表征揭示了CapI-vasa和CapI-nanos在多个体细胞组织中的表达，且表达模式基本重叠，但不完全相同。在卵裂的早期，两种转录本都被广泛表达;胃排空后，在推测的大脑，中胚层带和发育中的前肠中观察到表达。使用CapI-nanos和CapI-vasa作为标记，我们在幼虫中鉴定出推定的原始生殖细胞（PGC），它们最初以第4部分的小双侧簇形式出现，在幼虫后期以单个簇的形式出现。在成人中，第5段和第6段存在单个大的推定PGC簇。除了突出了两个滋养风虫之间这两个基因的表达谱差异外，我们还提出了关于Capitella sp中PGC起源和发育的假说。一世。

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