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首页> 外文期刊>Developmental dynamics: an official publication of the American Association of Anatomists >Toward understanding the morphogenesis of siliceous spicules in freshwater sponge: differential mRNA expression of spicule-type-specific silicatein genes in Ephydatia fluviatilis.
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Toward understanding the morphogenesis of siliceous spicules in freshwater sponge: differential mRNA expression of spicule-type-specific silicatein genes in Ephydatia fluviatilis.

机译：旨在了解淡水海绵中硅质针的形态：针状赤藓中针型特异性硅酸盐基因的差异mRNA表达。

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摘要

Siliceous spicules of sponges are morphologically diverse and provide good models for understanding the morphogenesis of biomineralized products. The silica deposition enzyme silicatein is a component of siliceous spicules of sponges and is thought to be the key molecule determining the morphology of spicules. Here, we focused on the silicateins of the freshwater sponge Ephydatia fluviatilis, which has two types of morphologically and functionally different spicules, called megascleres and gemmoscleres. We isolated six isoforms of silicateins and examined their mRNA expression in the cells producing megascleres and gemmoscleres. The spicule-type-specific mRNA expression of these isoforms and differential expression during spicule development suggest that the characteristic morphology of spicules is due to the specific properties and combinatory functions of silicatein isoforms.
机译：海绵的硅质针头在形态上是多种多样的，并为理解生物矿化产品的形态形成提供了良好的模型。二氧化硅沉积酶硅酸盐蛋白是海绵硅质针状体的成分，被认为是决定针状体形态的关键分子。在这里，我们集中研究淡水海绵埃菲达菌（Ephydatia fluviatilis）的硅酸盐，它具有两种形态和功能上不同的针状体，分别称为巨核和宝石。我们分离出六种亚型的硅酸盐蛋白，并检查了它们在产生巨细胞和宝石细胞的细胞中的mRNA表达。这些同工型的针型特定mRNA表达和针发育期间的差异表达表明，针的特征形态是由于硅酸盐同工型的特定性质和组合功能。

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