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首页> 外文期刊>Developmental immunology >A developmental bias in reading frame usage by human fetal thymic TCRBDJ transcripts is not present in genomic TCRBDJ rearrangements.
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A developmental bias in reading frame usage by human fetal thymic TCRBDJ transcripts is not present in genomic TCRBDJ rearrangements.

机译：基因组TCRBDJ重排中不存在人类胎儿胸腺TCRBDJ转录本在阅读框使用方面的发展偏见。

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摘要

We have previously reported that reading-frame usage and functional diversification is developmentally regulated, with virtually all TCRB DJ mRNA transcripts using a single reading frame at 8 weeks of gestational age, tapering to 50% by adult life. We used the polymerase chain reaction to create genomic libraries of DJ rearrangements in the TCRB locus from thymuses at 7.7, 10, and 16 weeks of gestational age, and from adult thymuses. Clones were randomly picked and sequenced to determine junctional sequences and reading-frame utilization. The resulting data address the hypothesis that cells bearing genomic joints in reading frame one are preferentially selected during fetal life. This hypothesis predicts that reading-frame bias would also be observed among genomic DJ joints. Instead, we observed random utilization of the three possible D-region reading frames among genomic D1s1 ==> J1s1 joints during fetal life. Similar results were obtained at 7.7 weeks of gestational age in a second thymus in which both RNA and DNA were simultaneously isolated and used to create libraries of TCRBDJ transcripts or rearrangements. We conclude that reading-frame utilization is random among genomic D1s1-JB1s1 rearrangements and that the preferential usage of a single reading frame among mRNA transcripts of TCRB DJ transcripts is the result of preferential transcription of genomic TCRB DJ joints in a single reading frame, or that TCRB DJ transcripts have a longer half-life than transcripts in reading frames two or three.
机译：我们以前曾报道过阅读框架的使用和功能多样化受到发展调节，几乎所有TCRB DJ mRNA转录本都在胎龄8周时使用单一阅读框架，到成年时逐渐减少到50％。我们使用聚合酶链反应在胎龄为7.7、10和16周的胸腺以及成年胸腺的TCRB基因座中创建DJ重排的基因组文库。随机选择克隆并测序以确定连接序列和阅读框利用率。所得数据解决了一个假设，即在胎儿生命期间优先选择在阅读框一中带有基因组接头的细胞。该假设预测在基因组DJ关节之间也会观察到阅读框偏倚。相反，我们观察到在胎儿生命期间，基因组D1s1 ==> J1s1关节中三个可能的D区域阅读框的随机利用。在第二个胸腺的妊娠期7.7周获得了相似的结果，其中同时分离了RNA和DNA，并用于创建TCRBDJ转录本或重排文库。我们得出结论，在基因组D1s1-JB1s1重排中阅读框利用是随机的，并且TCRB DJ转录本的mRNA转录本中单个阅读框的优先使用是基因组TCRB DJ接头在单个阅读框中优先转录的结果，或者TCRB DJ笔录的半衰期比阅读第二或第三帧的笔录更长。

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