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首页> 外文期刊>Developmental immunology >Induction of germinal centers by MMTV encoded superantigen on B cells.
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Induction of germinal centers by MMTV encoded superantigen on B cells.

机译：MMTV编码的超抗原在B细胞上诱导生发中心。

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It has not been established whether an endogenous superantigen (SAg) expressed on B cells can induce germinal centers (GCs). An interesting model is that of mammary tumor virus encoded viral SAgs, which induce vigorous T cell proliferation and are predominantly expressed on activated B cells. We have used this model to analyze the possibility that direct stimulation of Mtv7+ DBA/2 B cells by vSAg-responsive (Vbeta6+) BALB/c T cells can give rise to GCs. Injection of BALB/c SCID mice i.v. with 2 x 10(6) DBA/2 B cells, together with LPS, followed by 2 x 10(6) BALB/c T cells induces numerous large splenic GCs within 3-5 days. The GCs are still large on day 7, but are very much reduced by day 10. B cell activation with LPS is needed for this effect. These GCs form in spite of the apparent absence of follicular dendritic cells (FDCs) as judged by staining for several FDC surface markers. Control mice receiving either BALB/c T or DBA/2 B cells + LPS alone or DBA/2 T + B cells + LPS fail to exhibit any GCs on days 3-7. Numerous small clusters of PNA+ cells, but few large GCs are observed when TNF-R(p55)-Ig is also injected, whereas LTbetaR-Ig treatment impeded the formation of aggregations of these cells even further, leaving scattered PNA+ single cells and very small clumps throughout the white pulp of the spleens. Anti-TNFalpha had no effect. These results suggest that endogenous vSAg mediated GC formation is independent of antigen trapping by FDCs.
机译：尚未确定在B细胞上表达的内源性超抗原（SAg）是否可以诱导生发中心（GCs）。一个有趣的模型是乳腺肿瘤病毒编码的病毒SAg，它诱导剧烈的T细胞增殖并主要在活化的B细胞上表达。我们已经使用该模型分析了vSAg反应性（Vbeta6 +）BALB / c T细胞对Mtv7 + DBA / 2 B细胞的直接刺激会产生GC的可能性。静脉内注射BALB / c SCID小鼠。带有2个10（6）DBA / 2 B细胞，LPS和2个10（6）BALB / c T细胞的细胞在3-5天内诱导了许多大型脾脏GC。在第7天，GC仍然很大，但到第10天，GC的数量将大大减少。为达到这种效果，需要使用LPS激活B细胞。尽管通过对几种FDC表面标记进行染色判断，尽管明显不存在滤泡树突状细胞（FDC），但仍会形成这些GC。仅接受BALB / c T或DBA / 2 B细胞+ LPS或DBA / 2 T + B细胞+ LPS的对照小鼠在第3-7天没有表现出任何GC。 PNA +细胞有许多小的簇，但是当同时注射TNF-R（p55）-Ig时，观察到的大型GC很少，而LTbetaR-Ig处理则进一步阻止了这些细胞的聚集，留下了分散的PNA +单细胞，并且非常小在脾脏的白色浆液中结块。抗TNFα没有作用。这些结果表明，内源性vSAg介导的GC形成与FDC的抗原捕获无关。

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