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首页> 外文期刊>Diabetes research and clinical practice >Effects of antioxidants on glucose-induced oxidative stress and endoplasmic reticulum stress in endothelial cells.
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Effects of antioxidants on glucose-induced oxidative stress and endoplasmic reticulum stress in endothelial cells.

机译：抗氧化剂对葡萄糖诱导的内皮细胞氧化应激和内质网应激的影响。

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AIM: Hyperglycemia-induced endothelial cell dysfunction can be the result of increased oxidative stress and concomitant increase in endoplasmic reticulum (ER) stress. To test the extent of coupling between these two stresses, the effect of antioxidant vitamins on glucose-induced oxidative stress and ER stress in endothelial cells were studied. METHODS: Human umbilical vein endothelial cells (HUVEC) were treated with physiological (5.5mM) or supra-physiological (27.5mM) dextrose concentrations, and ER stress and oxidative stress were measured. Additional experiments were carried out in HUVEC over-expressing exogenous glucose transporter-1 (Glut-1) and treated with 5.5mM dextrose. RESULTS: Supra-physiological dextrose concentrations increased both ER stress and oxidative stress. However, while oxidative stress could be effectively inhibited with alpha-tocopherol and ascorbic acid, these antioxidants had no effect on ER stress. Increasing intracellular glucose levels by exogenous expression of Glut-1 in endothelial cells also increased oxidative stress and ER stress. Whereas the oxidative stress in these cells was reduced with alpha-tocopherol and ascorbic acid and dimethylsulfoxide, the ER stress could not be ameliorated with alpha-tocopherol and ascorbic acid. CONCLUSIONS: These results indicate that ER stress can be uncoupled from oxidative stress and antioxidants can ameliorate the latter without altering the ER stress induced by hyperglycemia.
机译：目的：高血糖引起的内皮细胞功能异常可能是氧化应激增加和内质网应激随之增加的结果。为了测试这两种压力之间的耦合程度，研究了抗氧化剂维生素对葡萄糖诱导的内皮细胞氧化应激和内质网应激的影响。方法：以生理浓度（5.5mM）或超生理浓度（27.5mM）的葡萄糖处理人脐静脉内皮细胞（HUVEC），并测定其内质网应激和氧化应激。在过表达HUVEC的外源葡萄糖转运蛋白1（Glut-1）中进行了其他实验，并用5.5mM葡萄糖处理。结果：超生理葡萄糖浓度增加内质网应激和氧化应激。但是，虽然可以使用α-生育酚和抗坏血酸有效地抑制氧化应激，但是这些抗氧化剂对ER应激没有影响。通过外源表达Glut-1在内皮细胞中增加细胞内葡萄糖水平也增加了氧化应激和内质网应激。尽管这些细胞的氧化应激通过α-生育酚，抗坏血酸和二甲基亚砜得以降低，但ER应激却不能通过α-生育酚和抗坏血酸得到缓解。结论：这些结果表明内质网应激可以与氧化应激分离，而抗氧化剂可以改善后者，而不会改变高血糖引起的内质网应激。

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