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首页> 外文期刊>Diatom Research >DIVERSITY OF PSEUDO-NITZSCHIA IN THE SOUTHEASTERN BAY OF BISCAY
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DIVERSITY OF PSEUDO-NITZSCHIA IN THE SOUTHEASTERN BAY OF BISCAY

机译：比斯开湾东南海岸的拟南芥多样性

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摘要

Ten species of the genus Pseudo-nitzschia were identified in the Nervion River estuary from preserved net tow samples and 31 strains isolated from the estuary. Species identification was performed by means of ultrastructural analysis of valve ornamentation and genetic analysis of selected strains of the P. delicatissima complex. Identified species include: P. australis, P. fraudulenta, P. pungens and P. subpacifica from the P. seriata complex, and P. arenysensis, P. galaxiae, P. multistriata, P. pseudodelicatissima and two uncertain P. pseudodelicatissima-like genotypes from the delicatissima complex. Most clonal strains corresponded to P. pseudodelicatissima-like taxa, P. fraudulenta and P. galaxiae. We were unable to identify the strains of the P.delicatissima-Yike, complex solely on the basis of morphology. A comparison of sequences of the rDNA ribosomal Internal Transcribed Spacers (ITS), including the regions ITS1, 5.8S and ITS2, of four strains of the P. delicatissima complex plus 23 sequences of taxonomically related strains available in GenBank, revealed the presence of P. arenysensis among strains of the P. delicatissima-morphologies and three different ITS types among the three P. pseudodelicatissima-like morphologies analysed. One corresponded to P. pseudodelicatissima sensu stricto (strain Ner-D5) whereas the other two (Ner-D6 and Ner-D8) constituted genetically distinct entities, which appeared in the phylogenetic tree as sister taxa to either P. cuspidata, the former, or to P. calliantha and P. mannii, the latter. The genetic differences among these three strains of P. pseudodelicatissima-like morphologies were corroborated by analysing their ITS2 secondary structure and comparing them with the ITS2 secondary structure of phylogenetically related species from GenBank.
机译：从保存的净丝束样品和从河口中分离出的31株菌株中，在Nervion河口中鉴定出10种假奈瑟菌属。通过瓣膜装饰的超微结构分析和对P. delicatissima复合体选定菌株的遗传分析进行物种鉴定。鉴定出的物种包括：来自P. seriata复合体的P. australis，P。欺诈性P. pungens和P. subpacifica，以及P. arenysensis，P。galaxiae，P。multistriata，P。pseudodelicatissima和两个不确定的P. pseudodelicatissima-like。 delicatissima复合体的基因型。大多数克隆毒株对应于拟假单胞菌样类群，欺诈性假单胞菌和galaxiae。我们无法仅根据形态来鉴定P.delicatissima-Yike菌株。比较了rDNA核糖体内部转录间隔区（ITS）的序列，包括四个P. delicatissima复合体菌株的ITS1、5.8S和ITS2区域，以及GenBank中提供的23种分类学相关菌株的序列，揭示了P的存在。分析了P. delicatissima形态的菌株之间的Arenysensis和三种P. pseudodelicatissima-like形态中的三种不同的ITS类型。一个对应于P. pseudodelicatissima sensu stricto（Ner-D5株），而另外两个对应于（Ner-D6和Ner-D8）构成遗传上不同的实体，它们在系统发育树中作为任一P. cuspidata的姐妹类群出现，或P. calliantha和P. mannii，后者。通过分析它们的ITS2二级结构并将其与GenBank的系统发育相关物种的ITS2二级结构进行比较，证实了这三种假单胞菌类形态菌株之间的遗传差异。

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