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Oilseed rape (Brassica napus L.) genotypic variation in responseto boron deficiency

机译:硼缺乏对油菜(甘蓝型油菜)基因型的影响

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Boron efficiency of 16 oilseed rape genotypes was tested using both hydroponic and pot-soil growing techniques. From the nutrient solution experiment (0.1 and 10 uM B), 4 representative contrasting genotypes were selected based on relative root and shoot growth. These were then grown in pots with low-B soil (0.25 mg kg~(-1)). From the nutrient solution experiment, 2 genotypes selected as B-inefficient did not show any growth disorders, and the concentration of B in the shoots was above critical. Furthermore, 4 contrasting genotypes were subjected to the stable UB isotope-enriched uptake solution for 6 h to verify possible low B-induced active uptake by roots and xylem loading of B. The concentration of UB in either root cell sap or xylem exudate washigher than in the external nutrient solution, which indicated the presence of low B-induced active uptake for all tested genotypes, and, to some extent, their efficiency with low B. In conclusion, a combination of different growing techniques under controlled environmental conditions together with different parameters including relative root and shoot weight, shoot B concentration, and B uptake provided reliable B efficiency results in oilseed rape genotypes.
机译:使用水培和盆栽土壤种植技术测试了16种油菜基因型的硼效率。从营养液实验(0.1和10 uM B)中,根据相对根和苗生长选择了4个代表性的对比基因型。然后将它们在低B土壤(0.25 mg kg〜(-1))的盆中生长。从营养液实验中,选择为B型低效率的2个基因型未显示任何生长障碍,并且芽中B的浓度超过临界值。此外,对4种不同的基因型进行了稳定的UB同位素富集的吸收溶液处理6 h,以验证可能的低B诱导的根部主动吸收和木质素的B吸收。根细胞汁液或木质部分泌物中UB的浓度高于在外部营养液中,表明所有测试基因型均存在低B诱导的主动摄取,并且在一定程度上表明其对低B的效率。总之,在受控的环境条件下,不同的生长技术与不同的组合包括相对根和苗重,芽B浓度和B吸收在内的参数为油菜油菜基因型提供了可靠的B效率结果。

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