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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation
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Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

机译：花粉辐照诱导鸢尾（Iris pseudacorus）中的单倍体植物

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This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. Anthers collected from the pollen donor were irradiated by X-ray with doses of 0, 100, 200, 300, and 400 Gy in 2012. A second round of experiments was conducted in 2013 with 300 and 400 Gy. The ovaries from each pollinated flower were collected and cultured and the produced plantlets were then acclimatized. Chromosome counting and flow cytometry were used to determine the ploidy levels. The viability of irradiated pollens was also evaluated. Pollination with irradiated pollen at doses ranging from 100 to 400 Gy can significantly reduce fruit set, particularly at higher doses (300 and 400 Gy). The embryo production was reduced in I. pseudacorus as doses increased; the highest doses led to reduction in the number of regenerants with the formation of just 6 and 4 embryos at 300 and 400 Gy, respectively. Haploid plantlets were produced when the pollen grains were exposed to 300 and 400 Gy of X-ray. Irradiation at 100 and 200 Gy was found to be insufficient to cause pollen sterility. The best dose for haploid production in I. pseudacorus is hereby found to be 300 or 400 Gy of X-ray.
机译：这是通过原位孤雌生殖继而进行胚胎抢救而成功开发的虹膜单倍体细胞系的成功报道。假性假单胞菌（2n = 38）的天然基因型是作为母本准备的，而丁香假单胞菌是男性。在2012年用0、100、200、300和400 Gy的剂量对从花粉供体收集的花药进行X射线照射。2013年进行了第二轮实验，分别使用300和400 Gy。收集并培养每朵授粉花的卵巢，然后使产生的小植株适应环境。使用染色体计数和流式细胞术确定倍性水平。还评估了辐照花粉的生存力。用100至400 Gy剂量的辐照花粉授粉可显着减少坐果，特别是在较高剂量（300和400 Gy）下。随着剂量的增加，假单胞菌的胚胎产量减少。最高剂量导致再生子数量减少，分别在300和400 Gy处仅形成6个和4个胚胎。当花粉粒暴露于300和400 Gy的X射线时，产生单倍体小苗。发现以100 Gy和200 Gy照射不足以引起花粉无菌。由此发现在假单胞菌中产生单倍体的最佳剂量是300或400Gy的X射线。

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