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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Somatic embryogenesis from immature cotyledons of apomictic and non-apomictic seeds in walnut (Juglans regia L.).
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Somatic embryogenesis from immature cotyledons of apomictic and non-apomictic seeds in walnut (Juglans regia L.).

机译：核桃（Juglans regia L.）的无融合生殖和非无融合生殖种子的子叶的体细胞胚发生。

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Laboratory experiments were conducted to study the somatic embryogenesis of immature cotyledons of apomictic and open-pollinated seeds in different walnut genotypes, i.e. Yalova-1, Sebin, Bilecik, KR-1, KR-2, Sen-2, 07-KOR-1, Tokat-1, Kaman-1 and Kaman-5. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen from apple cv. Golden Delicious. The best cotyledon stage for somatic embryogenesis was determined in open-pollinated seeds of the different walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after flowering. As a result, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after flowering. Driver and Kuniyuki walnut (DKW) medium supplemented with 1 mg benzyladenine, 2 mg kinetin, 0.01 mg IBA and 250 mg L-glutamine/litre was used in the initial cultures. Explants were then transferred to a DKW medium without growth regulators and L-glutamine in subcultures. The percentage of embryogenic cotyledons that originated from apomictic and non-apomictic seeds ranged from 3.6 to 25% and the number of embryos per cotyledon ranged from 1 to 9.7 at the end of the fourth subculture. A repetitively embryogenic embryo line originating from immature cotyledons of apomictic seeds of Tokat-1 was maintained by secondary embryogenesis..
机译：进行了实验室实验，以研究Yalova-1，Sebin，Bilecik，KR-1，KR-2，Sen-2、07-KOR-1等不同基因型的无融合生殖和开放授粉种子的未成熟子叶的体细胞胚发生。 ，Tokat-1，Kaman-1和Kaman-5。为了获得无融合生殖的种子，将雌花装袋和/或用苹果cv的花粉授粉。金美味。体细胞胚发生的最佳子叶阶段是在不同核桃基因型的开放授粉种子中确定的。开花后8、9、10、11和12周培养未成熟的子叶。结果，在开花后8周培养了被认为是无融合生殖来源的种子的子叶。在初始培养中使用添加了1毫克苄腺嘌呤，2毫克激动素，0.01毫克IBA和250毫克L-谷氨酰胺/升的Driver和Kuniyuki核桃（DKW）培养基。然后将外植体转移至不含有生长调节剂和L-谷氨酰胺的DKW培养基中。在第四次传代结束时，源自无融合生殖和非无融合生殖种子的胚性子叶的百分比为3.6％至25％，每个子叶的胚数为1％至9.7％。通过二次胚胎发生维持源自Tokat-1无融合生殖种子的不成熟子叶的重复胚发生胚胎系。

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