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首页> 外文期刊>Turkish journal of biology >Use of Gamma Rays to Induce Mutations in Four Pea (Pisum sativum L.) Cultivars
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Use of Gamma Rays to Induce Mutations in Four Pea (Pisum sativum L.) Cultivars

机译：利用γ射线诱导四个豌豆（Pisum sativum L.）品种的突变

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The study reports a 3-step optimization to find out the effects of cytokinins BAP and TDZ on seed germination and g rays to induce mutations in 4 pea cultivars: Winner, Sprinter, Bolero and Karina. It was observed that germination was independent of the dose of g rays and was mainly affected by the germination capability of the genotypes and doses of BAP and TDZ in the germination medium. Seed germination was better on germination medium containing 50 μM BAP than 10 μM TDZ. Variable rooting was observed on shoots obtained from non-irradiated seeds germinated on MS medium containing 50 μM BAP. However, shoots of 2 cv. Winner irradiated with 60 Gy and Karina irradiated with 140 Gy g rays and germinated on 50 μM BAP showed reduced rooting. Abbreviations: g radiations: gamma radiations, BAP: 6 Benzylaminopurine, TDZ: Thidiazuron, NAA: a Naphthalene acetic acid, IBA: Indole 3 butyric acid.
机译：该研究报告进行了三步优化，以找出细胞分裂素BAP和TDZ对种子萌发和g射线的影响，以诱导4个豌豆品种的突变：Winner，Sprinter，Bolero和Karina。观察到发芽与g射线的剂量无关，并且主要受基因型的发芽能力以及BAP和TDZ在发芽培养基中的剂量的影响。在含有50μMBAP的发芽培养基上，种子发芽比10μMTDZ更好。从含有50μMBAP的MS培养基上发芽的未辐照种子获得的芽上观察到可变的生根。然而，芽2 cv。用60 Gy照射的优胜者和用140 Gy g射线照射并在50μMBAP上发芽的Karina表现出减少的生根。缩写：g射线：γ射线，BAP：6苄基氨基嘌呤，TDZ：噻二隆，NAA：萘乙酸，IBA：吲哚3丁酸。

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