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首页> 外文期刊>Turkish journal of biology >Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture
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Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture

机译：在鳞茎鳞茎培养中诱导贝母形态发生过程中抗氧化酶的活性

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摘要

We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L~(-1) thidiazuron. Bulbs were grown at standard (24 ℃) and low temperatures (4 ℃) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in order to examine the influence of the above-mentioned pretreatments and nutrient medium composition on enzyme activity. Enzyme activity was measured 7, 14, 21, and 28 days after the start of morphogenesis induction. Superoxide dismutase and catalase showed the highest activity at the beginning of morphogenesis in vitro. Peroxidase activity was the highest in bulb segments immediately after isolation following pretreatments and on both nutrition media. We also determined the effect of pretreatment coupled with low temperature (4 ℃) on growth and development of F. meleagris in vitro and chlorophyll and carotenoids content.
机译：我们调查了鳞状贝母体外培养中贝母形态发生过程中三种抗氧化酶的活性：超氧化物歧化酶，过氧化氢酶和过氧化物酶。在添加了1.0 mg L〜（-1）噻二氮酮的营养培养基上建立了小菜豆鳞茎的培养。在开始的前6周（预处理）中，鳞茎在标准温度（24℃）和低温（4℃）或蔗糖浓度增加（4.5％）下生长。然后将所有鳞茎在标准温度下于2、4-二氯苯氧基乙酸和激动素或噻唑酮（每种浓度为1 mg L-1）上生长4周，以检查上述预处理和营养培养基组成对酶活性。在形态发生诱导开始后第7、14、21和28天测量酶活性。超氧化物歧化酶和过氧化氢酶在体外形态发生开始时表现出最高的活性。预处理后立即分离的球茎段和两种营养培养基中的过氧化物酶活性最高。我们还确定了低温（4℃）联合预处理对拟南芥生长和发育以及叶绿素和类胡萝卜素含量的影响。

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