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首页> 外文期刊>Turkish journal of biology >Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012
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Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

机译：2008-2012年土耳其爆发的小反刍兽疫病毒的系统发育分析

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摘要

Peste des petits ruminants (PPR) is an important viral disease of sheep and goats and is endemic in all regions of Turkey. In this study, PPR virus infection was investigated by RT-PCR assay based on the fusion (F) gene in PPR-suspected sheep and goat samples. PPR virus RNA was detected in 65 small ruminants (51 sheep, 14 goats) from independent outbreaks during 2008–2012 in provinces in the central and Mediterranean regions and the central-west part of the Aegean region in Turkey. The virus was detected in an aborted sheep fetus sample by RT-PCR, and diagnosis was also confirmed by virus isolation. Vaccine strain Nigeria 75/1 was differentiated from field isolates by restriction fragment length polymorphism analysis of RT-PCR products using EcoRI. Phylogenetic analysis of 16 viruses indicated that all viruses, including the one from the aborted sheep fetus, belonged to lineage IV, as had the PPR viruses previously isolated in Turkey. Nucleotide sequence identity among 16 viruses was 99.1%–100%. Results showed that PPR virus lineage IV has been in circulation in Turkey since the first detection of the disease.
机译：小反刍兽疫是绵羊和山羊的重要病毒性疾病，在土耳其所有地区都很流行。在这项研究中，通过RT-PCR检测基于PPR怀疑的绵羊和山羊样本中的融合（F）基因，研究了PPR病毒感染。在2008-2012年间，在土耳其中部和地中海地区以及爱琴海地区的中西部地区的独立暴发中，在65只小反刍动物（51只绵羊，14只山羊）中检测到PPR病毒RNA。通过RT-PCR在流产的绵羊胎儿样品中检测到该病毒，并通过病毒分离也证实了诊断。通过使用EcoRI对RT-PCR产物进行限制性片段长度多态性分析，从现场分离株中分离出了尼日利亚菌株75/1。对16种病毒的系统发育分析表明，所有病毒，包括来自流产的绵羊胎儿的一种病毒，都属于IV谱系，与先前在土耳其分离的PPR病毒一样。 16种病毒之间的核苷酸序列同一性为99.1％–100％。结果表明，自从首次发现该病以来，PPR病毒谱系IV就一直在土耳其流通。

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