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首页> 外文期刊>Turkish journal of biology >Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis
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Nitrate and iron nutrition effects on some nitrate assimilation enzymes and metabolites in Spirulina platensis

机译：硝酸盐和铁营养对螺旋藻某些硝酸盐同化酶和代谢产物的影响

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摘要

Effects of different sodium nitrate and iron concentrations on the production of some metabolites and pigments and the activities of some nitrate assimilation enzymes [nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT)] were investigated in the growth medium of Spirulina platensis (Gamont) Geitler. For this purpose, S. platensis was cultivated on Zarrouk’s medium containing different sodium nitrate concentrations (10–180 mM). A 100 mM sodium nitrate concentration stimulated growth of this organism as well as the production of pigments and other metabolites and enzyme activities. In the presence of 100 mM sodium nitrate, different concentrations of iron (10–100 μM) were tried in the growth medium of S. platensis. The highest enzyme activities were determined in the presence of 100 mM sodium nitrate and 50 μM iron. The highest NR, NiR, GS, and GOGAT activities obtained were 126.92 ± 9.2 U mL~(–1), 841.16 ± 61.4 U mL~(–1), 0.1301 ± 0.02 U mL~(–1), and 46.18 ± 1.8 U mL~(–1), respectively. These high enzymatic activities may stimulate high amino acid production. Higher enzyme activity may result in higher nutritional value in S. platensis, which has wide usage in biotechnology, industry, and biochemistry.
机译：不同浓度的硝酸钠和铁对某些代谢产物和色素的产生以及某些硝酸盐同化酶[硝酸还原酶（NR），亚硝酸还原酶（NiR），谷氨酰胺合成酶（GS）和谷氨酸合成酶（GOGAT）]活性的影响在螺旋藻（Gamont）盖特勒的生长培养基中进行了研究。为此，在含有不同硝酸钠浓度（10-180 mM）的Zarrouk培养基上培养了S. platensis。浓度为100 mM的硝酸钠刺激了该生物的生长以及色素和其他代谢产物的产生以及酶的活性。在存在100 mM硝酸钠的情况下，尝试在S. platensis的生长培养基中使用不同浓度的铁（10-100μM）。在100 mM硝酸钠和50μM铁的存在下测定了最高的酶活性。获得的最高NR，NiR，GS和GOGAT活性分别为126.92±9.2 U mL〜（–1），841.16±61.4 U mL〜（–1），0.1301±0.02 U mL〜（–1）和46.18±1.8分别为U mL〜（–1）。这些高酶活性可能刺激高氨基酸产量。较高的酶活性可能会导致白僵菌的营养价值更高，而白僵菌在生物技术，工业和生物化学中都有广泛的用途。

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