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首页> 外文期刊>Turkish journal of biology >Effect of gamma ray irradiation and cryopreservation on pollen stainability, in vitro germination, and fruit set in Citrus
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Effect of gamma ray irradiation and cryopreservation on pollen stainability, in vitro germination, and fruit set in Citrus

机译：γ射线辐照和低温保存对柑橘花粉染色性，离体萌发和坐果的影响

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摘要

The present work analyzed the stainability and in vitro germinability of the gamma-ray-irradiated fresh and cryopreserved pollens of 3 Citrus species: C. limon, C. limetta, and C. sinensis. Fruit set after pollinating 75 flowers of C. grandis with irradiated and nonirradiated (control) fresh and cryopreserved pollen was also assessed. Results showed that in solid and liquid culture media the highest in vitro germination values were detected in fresh (61.45% and 62.53%, respectively) as well as cryopreserved (53.17% and 55.36%, respectively) C. sinensis nonirradiated pollens. However, at a higher irradiation dose of 500 Gy in both culture media, the minimum reduction of germinability of fresh (30.85% and 28.01%) and cryopreserved pollens (33.37% and 31.06%) was found in C. limon. Contrary to in vitro germination, the minimum reduction in stainability of fresh and cryopreserved pollens, as assessed by acetocarmine (9.51% and 11.85%, respectively) and FDA tests (10.78% and 12.73%, respectively), was recorded in C. limetta. Regardless of irradiation dose, the highest fruit setting in C. grandis at 40 days after pollination was detected when it was pollinated with both fresh (38.02%) and cryopreserved (34.92%) C. limetta pollen grains.
机译：本工作分析了3种柑橘属柠檬（C. limon，C。limetta和C. sinensis）的伽马射线辐照的新鲜和冷冻保存的花粉的可染性和体外萌发能力。还评估了用辐照和未辐照（对照）新鲜和冷冻保存的花粉对75朵大花茶授粉后的坐果。结果表明，在固态和液态培养基中，新鲜（分别为61.45％和62.53％）和冷冻保存（分别为C. sinensis）未辐照花粉的体外萌发值最高。然而，在两种培养基中以500 Gy的较高辐照剂量时，柠檬柠檬梭菌的新鲜花粉（30.85％和28.01％）和冷冻保存的花粉的发芽率最低降低。与体外发芽相反，新鲜的和冷冻保存的花粉的可染性最低降低，是通过乙酰胭脂红（分别为9.51％和11.85％）和FDA测试（分别为10.78％和12.73％）评估的，被记录在石灰梭菌中。不论辐照剂量如何，当用新鲜（38.02％）和冷冻保存（34.92％）的柠条花粉花粉授粉时，在授粉后第40天都可检测到最高的坐果。

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