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首页> 外文期刊>Turkish journal of biology >Study of exogenous oxidative stress response in Escherichia coli, Pseudomonas spp., Bacillus spp., and Salmonella spp.
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Study of exogenous oxidative stress response in Escherichia coli, Pseudomonas spp., Bacillus spp., and Salmonella spp.

机译：研究大肠杆菌，假单胞菌，芽孢杆菌和沙门氏菌中的外源氧化应激反应。

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With a previous observation of Escherichia coli growth cessation with the supplementation of 3 mM hydrogen peroxide (H_2O_2) at the late log phase, the current study further demonstrated the consequences of the addition of an increased concentration (6 mM) of H_2O_2 and further extended the investigation on such an oxidant's impact on the growth of Salmonella spp., Pseudomonas spp., and Bacillus spp. Cell culturability was measured through the enumeration of colony-forming units (CFUs) on agar plates for up to 72 h. Subsequent changes in cell morphology and arrangements were monitored, and the cell viability was simultaneously retraced by spot tests. A sharp decline in the culturable cells of E. coli was observed after 48 h with a large mass of cell aggregates upon addition of H_2O_2, while Pseudomonas spp. lost viability after 36 h. Impaired morphology of such stressed cells was comparable to those of the untreated cells. Notably, Pseudomonas cells were more prone to oxidative damage compared to E. coli. In contrast, the impact of H_2O_2 was insignificant in the case of Salmonella spp. and Bacillus spp., suggestive of a stringent defense mechanism against oxidative stress.
机译：先前的观察表明，在对数后期后期补充3 mM过氧化氢（H_2O_2）会导致大肠杆菌停止生长，本研究进一步证明了增加浓度（6 mM）的H_2O_2的后果，并进一步扩大了研究这种氧化剂对沙门氏菌，假单胞菌和芽孢杆菌的生长的影响。通过在琼脂平板上计数菌落形成单位（CFU）长达72小时来测量细胞的可培养性。监测细胞形态和排列的后续变化，并通过斑点测试同时追溯细胞活力。加入H_2O_2后48 h观察到E. coli可培养细胞急剧下降，大量细胞聚集，而Pseudomonas spp。 36小时后丧失生存能力。这种应激细胞的形态受损与未经处理的细胞相当。值得注意的是，与大肠杆菌相比，假单胞菌细胞更容易受到氧化损伤。相反，对于沙门氏菌，H_2O_2的影响微不足道。和芽孢杆菌属（Bacillus spp。），暗示了针对氧化应激的严格防御机制。

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