...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Developmental cell >P37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis
【24h】

P37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis

机译：P37是p97衔接子，在相间和有丝分裂结束时高尔基体和ER生物发生

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We previously reported that p97/p47-assisted membrane fusion is important for the reassembly of organelles at the end of mitosis, but not for their maintenance during interphase. We have now identified a p97 adaptor protein, p37, which forms a complex with p97 in the cytosol and localizes to the Golgi and ER. siRNA experiments revealed that p37 is required for Golgi and ER biogenesis. Injection of anti-p37 antibodies into cells at different cell cycle stages showed that p37 plays an important role in both Golgi and ER maintenance during interphase as well as in their reassembly at the end of mitosis. In an in vitro Golgi reassembly assay, the p97/p37 complex has membrane fusion activity. In contrast to the p97/p47 pathway, this pathway requires p115-GM130 tethering and SNARE GS15, but not syntaxin5. Interestingly, although VCIP135 is also required, its deubiquitinating activity is unnecessary for p97/p37-mediated activities.
机译：我们先前曾报道过p97 / p47辅助的膜融合对于有丝分裂末期的细胞器的重组非常重要，但对于中间期的维持却不重要。现在我们已经鉴定出p97衔接子蛋白p37，它在细胞质中与p97形成复合物，并定位于高尔基体和内质网。 siRNA实验表明，p37是高尔基体和ER生物发生所必需的。在不同细胞周期阶段向细胞中注射抗p37抗体表明，p37在中间相期间的高尔基体和ER维持中以及在有丝分裂结束时的重组中都起着重要作用。在体外高尔基体重组试验中，p97 / p37复合物具有膜融合活性。与p97 / p47途径相反，该途径需要p115-GM130系留和SNARE GS15，但不需要syntaxin5。有趣的是，尽管也需要VCIP135，但对于p97 / p37介导的活性，其去泛素化活性是不必要的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号