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首页> 外文期刊>Digestive Diseases and Sciences >Long-term repopulation effects of donor BMDCs on intestinal epithelium.
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Long-term repopulation effects of donor BMDCs on intestinal epithelium.

机译：供体BMDC对肠道上皮细胞的长期繁殖作用。

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BACKGROUND: Bone marrow-derived cells (BMDCs) have the ability to differentiate into intestinal epithelial cells after transplantation and participate in the regeneration process of damaged epithelium. AIMS: To investigate whether BMDCs could differentiate into intestinal epithelium long term in chimeric mice after transplantation and without special treatment. METHODS: Forty irradiated C57BL/6 mice were used. Thirty of them (group A) received transplantation of BMDCs from GFP transgenic mice, and ten (group B) received PBS. The chimeric percentage at the 14th month was examined by flow cytometry. Engraftment of BMDCs was detected by immunohistochemistry in intestinal epithelium. Immunofluorescence observation was used to detect coexpression of PCK, CD45 and Chromogranin A with GFP. BMDCs in the epithelium were observed by an immune electron microscope. The percent of GFP(+) epithelial cells was also determined by flow cytometry. RESULTS: Mice in group A had a survival rate of 93.3% 1 week after transplantation. BMDCs could engraft into recipients' intestinal epithelium. These cells expressed epithelial cell marker PCK, but could not express CD45. Some of them differentiated into enteroendocrine cells expressing Chromogranin A. GFP(+) villous epithelial cells ranged from 9.41 to 16.07% in different subgroups of group A. BMDCs in epithelium developed the characteristics of enterocytes and goblet cells. GFP(+)/PCK(+) epithelial cells at the 6th month made up a proportion of 16.11% among all the isolated epithelial cells. CONCLUSIONS: Long term, BMDCs could repopulate recipient's intestinal epithelium even without any special treatment, which suggests a novel insight into the maintenance of the intestinal epithelial constitution.
机译：背景：骨髓来源的细胞（BMDCs）具有移植后分化为肠上皮细胞的能力，并参与受损上皮的再生过程。目的：研究在没有特殊治疗的情况下，BMDCs能否在嵌合小鼠中长期分化为肠上皮细胞。方法：使用40只经辐照的C57BL / 6小鼠。其中三十只（A组）接受了GFP转基因小鼠的BMDC移植，十只（B组）接受了PBS。通过流式细胞术检查第14个月的嵌合百分比。通过免疫组织化学在肠上皮中检测到BMDC的植入。免疫荧光观察用于检测PCK，CD45和嗜铬粒蛋白A与GFP的共表达。通过免疫电子显微镜观察上皮中的BMDC。 GFP（+）上皮细胞的百分比也通过流式细胞仪确定。结果：A组小鼠移植后1周生存率为93.3％。 BMDC可以植入受体的肠上皮。这些细胞表达上皮细胞标记物PCK，但不表达CD45。它们中的一些分化成表达嗜铬粒蛋白A的肠内分泌细胞。A组不同亚组的GFP（+）绒毛状上皮细胞在9.41％至16.07％之间。上皮中的BMDCs具有肠上皮细胞和杯状细胞的特征。在所有分离的上皮细胞中，第6个月的GFP（+）/ PCK（+）上皮细胞占16.11％的比例。结论：长期而言，即使没有任何特殊治疗，BMDCs仍可以使受体的肠上皮重新填充，这表明对维持肠上皮结构有新的认识。

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