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首页> 外文期刊>Digestive Diseases and Sciences >Application of tissue microarrays to study the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis.
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Application of tissue microarrays to study the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis.

机译:应用组织芯片研究地塞米松对重症急性胰腺炎大鼠胰腺NF-κB表达的影响。

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摘要

To discuss the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-kappaB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-kappaB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-kappaB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.
机译:探讨地塞米松对重症急性胰腺炎(SAP)大鼠胰腺NF-κB表达的影响。将90只大鼠SAP模型分为模型组和地塞米松治疗组,每组45只。选择健康大鼠45只作为假手术组。将这些组分为3、6和12小时组,每组15只大鼠。术后3、6、12 h观察生存期,胰腺病理变化。观察胰腺组织微阵列中NF-κB蛋白表达水平的变化。治疗组在3和6 h时的胰腺病理评分显着低于模型组(P <0.05),在12 h时显着低于模型组(P <0.01)。 3h时,治疗组胰头NF-κB蛋白的表达水平明显低于模型组(P <0.01)。地塞米松在SAP期间减轻胰腺组织损伤可能与其在抑制NF-κB表达和调节细胞因子中的作用密切相关。组织微阵列在胰腺炎病理检查中的优势包括节省时间和能源,高效和具有代表性的结果。

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