• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>DNA research: an international journal for rapid publication of reports on genes and genomes >Identifying the genome-wide genetic variation between precocious trifoliate orange and its wild type and developing new markers for genetics research
【24h】

Identifying the genome-wide genetic variation between precocious trifoliate orange and its wild type and developing new markers for genetics research

机译：识别早熟三叶橙与其野生型之间的全基因组遗传变异，并为遗传学研究开发新的标记

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To increase our understanding of the genes involved in flowering in citrus, we performed genome resequencing of an early flowering trifoliate orange mutant (Poncirus trifoliata L. Raf.) and its wild type. At the genome level, 3,932,628 single nucleotide polymorphisms (SNPs), 1,293,383 insertion/deletion polymorphisms (InDels), and 52,135 structural variations were identified between the mutant and its wild type based on the citrus reference genome. Based on integrative analysis of resequencing and transcriptome analysis, 233,998 SNPs and 75,836 InDels were also identified between the mutant and its wild type at the transcriptional level. Also, 272 citrus homologous flowering-time transcripts containing genetic variation were also identified. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes annotation revealed that the transcripts containing the mutant- and the wild-type-specific InDel were involved in diverse biological processes and molecular function. Among these transcripts, there were 131 transcripts that were expressed differently in the two genotypes. When 268 selected InDels were tested on 32 genotypes of the three genera of Rutaceae for the genetic diversity assessment, these InDel-based markers showed high transferability. This work provides important information that will allow a better understanding of the citrus genome and that will be helpful for dissecting the genetic basis of important traits in citrus.
机译：为了增加对柑橘开花相关基因的了解，我们对早期开花的三叶橙色突变体（Poncirus trifoliata L. Raf。）及其野生型进行了基因组重测序。在基因组水平上，基于柑橘类参考基因组，在突变体及其野生型之间鉴定出3,932,628个单核苷酸多态性（SNP），1,293,383个插入/缺失多态性（InDels）和52,135个结构变异。基于重测序和转录组分析的综合分析，在转录水平上，在突变体与其野生型之间还鉴定出233,998个SNP和75,836个InDel。此外，还鉴定了272个含有遗传变异的柑橘同源开花时间转录本。基因本体论和京都基因与基因组百科全书注释显示，含有突变型和野生型特异性InDel的转录本参与了多种生物过程和分子功能。在这些转录本中，有131个转录本在两种基因型中表达不同。当对芸香科三个属的32个基因型测试268个选定的InDel进行遗传多样性评估时，这些基于InDel的标记显示出很高的可转移性。这项工作提供了重要的信息，将有助于人们更好地了解柑橘的基因组，并有助于剖析柑橘重要性状的遗传基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号