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首页> 外文期刊>DNA and Cell Biology >Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate-Induced DNA Damage in Mice Organs
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Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate-Induced DNA Damage in Mice Organs

机译：番红花柱头提取物和crocro（trans-crocin 4）对甲磺酸甲酯诱导的小鼠器官DNA损伤的保护作用

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This study was designed to examine the effect of aqueous extract of Crocus sativus stigmas (CSE) and crocin (trans-crocin 4) on methyl methanesulfonate (MMS)-induced DNA damage in multiple mice organs using the comet assay. Adult male NMRI mice in different groups were treated with either physiological saline (10 mL/Kg, intraperitoneal [ip]), CSE (80 mg/Kg, ip), crocin (400 mg/Kg, ip), MMS (120 mg/Kg, ip), and CSE (5, 20, and 80 mg/Kg, ip) 45 min prior to MMS administration or crocin (50, 200, and 400 mg/Kg, ip) 45 min prior to MMS administration. Mice were sacrificed about 3 h after each different treatment, and the alkaline comet assay was used to evaluate the effect of these compounds on DNA damage in different mice organs. The percent of DNA in the comet tail (% tail DNA) was measured. A significant increase in the % tail DNA was seen in nuclei of different organs of MMS-treated mice. In control groups, no significant difference was found in the % tail DNA between CSE- or crocin-pretreated and saline-pretreated mice. The MMS-induced DNA damage in CSE-pretreated mice (80 mg/Kg) was decreased between 2.67-fold (kidney) and 4.48-fold (lung) compared to those of MMS-treated animals alone (p < 0.001). This suppression of DNA damage by CSE was found to be depended on the dose, which pretreatment with CSE (5 mg/Kg) only reduced DNA damage by 6.97%, 6.57%, 7.27%, and 9.90% in liver, lung, kidney, and spleen, respectively (p > 0.05 as compared with MMS-treated group). Crocin also significantly decreased DNA damage by MMS (between 4.69-fold for liver and 6.55-fold for spleen, 400 mg/Kg), in a dose-dependent manner. These data indicate that there is a genoprotective property in CSE and crocin, as revealed by the comet assay, in vivo.
机译：这项研究的目的是使用彗星试验检测番红花番红花（CSE）和番红花（反式番红花4）的水提物对甲烷磺酸甲酯（MMS）诱导的多个小鼠器官DNA损伤的影响。用生理盐水（10 mL / Kg，腹膜内[ip]），CSE（80 mg / Kg，ip），crocin（400 mg / Kg，ip），MMS（120 mg /服用MMS之前45分钟（Kg，ip）和CSE（5、20和80 mg / Kg，ip）或服用MMS之前45分钟的番红花（50、200和400 mg / Kg，ip）。每次不同的治疗后约3小时处死小鼠，并使用碱性彗星试验评估这些化合物对不同小鼠器官DNA损伤的影响。测量彗星尾巴中的DNA百分比（尾巴DNA百分比）。在MMS处理的小鼠的不同器官的细胞核中发现尾巴DNA的百分比显着增加。在对照组中，经CSE或番红花预处理和盐水预处理的小鼠的尾巴DNA百分比无显着差异。与单独使用MMS的动物相比，在CSE预处理的小鼠（80 mg / Kg）中，MMS诱导的DNA损伤降低了2.67倍（肾脏）至4.48倍（肺）（p <0.001）。发现CSE对DNA损伤的这种抑制作用取决于剂量，用CSE预处理（5 mg / Kg）只能使肝，肺，肾中的DNA损伤减少6.97％，6.57％，7.27％和9.90％，和脾脏（与MMS治疗组相比，p> 0.05）。番红花还以剂量依赖的方式显着降低了MMS对DNA的损伤（肝脏为4.69倍，脾为6.55倍，400 mg / Kg）。这些数据表明，如彗星试验所揭示的，在体内CSE和crocrocin具有遗传保护作用。

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