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首页> 外文期刊>DNA and Cell Biology >Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer.
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Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer.

机译：游离循环肿瘤DNA的定量作为乳腺癌的诊断标志物。

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AIM: To determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR quantification methodology. METHODS: Our standard protocol for quantification of cell-free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. RESULTS: We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs. 77.06 ng/mL, p 0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery (105.2 vs. 59.0 ng/mL, p = 0.001). Increased plasma cell-free DNA concentration was a strong risk factor for breast cancer, conferring an increased risk for the presence of this disease (OR, 12.32; 95% CI, 2.09-52.28; p 0.001). CONCLUSIONS: Quantification of circulating DNA by real-time PCR may be a good and simple tool for detection of breast cancer with a potential to clinical applicability together with other current methods used for monitoring the disease.
机译：目的：通过实时PCR定量方法确定循环DNA的量是否能区分乳腺癌患者和健康个体。方法：我们的定量无细胞血浆DNA的标准方案涉及175位连续的乳腺癌患者和80位健康对照。结果：我们发现与对照组相比，乳腺癌患者的循环DNA水平升高（105.2对77.06 ng / mL，p <0.001）。我们还发现，乳腺癌手术前后患者的循环DNA量存在统计学差异（105.2 vs. 59.0 ng / mL，p = 0.001）。血浆无细胞DNA浓度升高是乳腺癌的重要危险因素，导致存在该疾病的风险增加（OR，12.32; 95％CI，2.09-52.28; p <0.001）。结论：通过实时PCR定量循环DNA可能是检测乳腺癌的一种好工具，并且具有与其他当前用于监测疾病的方法相结合的潜在临床应用价值。

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