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首页> 外文期刊>Dynamic Soil, Dynamic Plant >Molecular Characterization of Azotobacter chroococcum Strains Isolated from Different Agro-climatic Zones of Karnataka, India
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Molecular Characterization of Azotobacter chroococcum Strains Isolated from Different Agro-climatic Zones of Karnataka, India

机译：从印度卡纳塔克邦不同农业气候区分离的嗜蓝固氮菌的分子表征

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摘要

Azotobacter chroococcum is an aerobic, free-living bacterium capable of synthesizing various plant growth-promoting substances and is useful in crop improvement for biological nitrogen-fixing activity. Ten A. chroococcum strains were isolated and purified from different agro-climatic zones of Karnataka, India by Waksman No. 77 N-free agar by a serial dilution plate technique. Molecular diversity of the isolates was estimated by using ten selected RAPD primers. A total of 103 bands were scored out ofwhich 87 were found to be polymorphic (84.97%). A dendrogram divided the isolates into two groups separated by 37 linkage distances and the dissimilarity matrix showed a maximum difference of 64% between the isolates of the North eastern transition zoneand the Central dry zone and a minimum difference of 18% between the isolates of the Eastern dry zone and the Hilly zone. The isolate from zone 4 was clustered separately from the group. Thus, RAPD markers analysis proved to be a quick, simple and significant testing method to assess genetic diversity among A. chroococcum isolates.
机译：绿偶氮细菌是一种有氧，自由生活的细菌，能够合成各种促进植物生长的物质，可用于作物改良中的生物固氮活性。通过连续稀释平板技术，用Waksman No. 77 N-N琼脂从印度卡纳塔克邦的不同农业气候区中分离和纯化了十种嗜酸链球菌菌株。通过使用十种选定的RAPD引物评估分离株的分子多样性。共计103条谱带，其中87条具有多态性（84.97％）。通过树状图将分离株分为以37个连锁距离隔开的两组，相异矩阵显示东北过渡区和中央干旱区的分离株之间最大差异为64％，东部过渡区的分离株之间最小差异为18％干旱区和丘陵区。来自区域4的分离物与该组分开聚类。因此，RAPD标记分析被证明是一种快速，简单且重要的测试方法，可用于评估嗜铬曲霉分离株之间的遗传多样性。

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